Rabbit anti-Human RNF168 Polyclonal Antibody | anti-RNF168 antibody

RNF168, CT (RNF168, E3 ubiquitin-protein ligase RNF168, RING finger protein 168) (APC)

Cat. Num. AAA6342710

• Gene Names: RNF168; hRNF168
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF168; Polyclonal Antibody; CT (RNF168; E3 ubiquitin-protein ligase RNF168; RING finger protein 168) (APC); RING finger protein 168; anti-RNF168 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RNF168 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RNF168 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467-496 amino acids from the C-terminal region of human RNF168.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RNF168 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF168 antibody detected the RNF168 protein (arrow).)

Related Product Information for anti-RNF168 antibody

The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).

Product Categories/Family for anti-RNF168 antibody

Antibodies; Ubiquitin Ligases

References

Lilley, C.E., et al. EMBO J. 29(5):943-955(2010). Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010). Ramachandran, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):809-814(2010). Doil, C., et al. Cell 136(3):435-446(2009). Stewart, G.S., et al. Cell 136(3):420-434(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31377566
• NCBI GeneID: 165918
• NCBI Accession #: NP_689830.2
• NCBI GenBank Nucleotide #: NM_152617.3
• UniProt Accession #: Q8IYW5; Q8NA67; Q96NS4
• Molecular Weight: 65,020 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF168
• NCBI Official Synonym Full Names: ring finger protein 168, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF168
• NCBI Official Synonym Symbols: hRNF168
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF168
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF168
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF168
• UniProt Synonym Gene Names: hRNF168
• UniProt Entry Name: RN168_HUMAN

Uniprot Description

RNF168: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8- dependent H2A ubiquitination, promoting the formation of 'Lys-63'- linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively). Defects in RNF168 are the cause of Riddle syndrome (RIDDLES). Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions. Belongs to the RNF168 family.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: ubiquitin binding; protein binding; histone binding; zinc ion binding; nucleosome binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of DNA repair; double-strand break repair; isotype switching; protein ubiquitination; response to ionizing radiation; response to DNA damage stimulus

Disease: Riddle Syndrome