Rabbit anti-Human RNF14 Polyclonal Antibody | anti-RNF14 antibody

RNF14, CT (RNF14, ARA54, E3 ubiquitin-protein ligase RNF14, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein) (FITC)

Cat. Num. AAA6342602

• Gene Names: RNF14; ARA54; HFB30; TRIAD2; HRIHFB2038
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF14; Polyclonal Antibody; CT (RNF14; ARA54; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein) (FITC); Triad2 protein; anti-RNF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 474

• Applicable Applications for anti-RNF14 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RNF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-364 amino acids from the C-terminal region of human RNF14.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNF14 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RNF14 antibody detected the RNF14 protein (arrow).)

Related Product Information for anti-RNF14 antibody

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Product Categories/Family for anti-RNF14 antibody

Antibodies; Ubiquitin Ligases

References

Xu, K., et al. Cancer Cell 15(4):270-282(2009). Lan, K.C., et al. Fertil. Steril. 89 (5 SUPPL), 1397-1405 (2008): Kikuchi, H., et al. Carcinogenesis 28(8):1752-1758(2007). Yang, Z., et al. Endocrinology 148(3):1340-1349(2007). Yang, Z., et al. Mol. Endocrinol. 21(2):343-358(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 34577098
• NCBI GeneID: 9604
• NCBI Accession #: NP_899646.1; NP_004281.1
• NCBI GenBank Nucleotide #: NP_899646.1
• UniProt Accession #: Q9UBS8
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF14 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 14
• NCBI Official Symbol: RNF14
• NCBI Official Synonym Symbols: ARA54; HFB30; TRIAD2; HRIHFB2038
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF14
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF14
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF14
• UniProt Synonym Gene Names: ARA54
• UniProt Entry Name: RNF14_HUMAN

NCBI Description

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]

Uniprot Description

RNF14: Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. Belongs to the RBR family. RNF14 subfamily.

Protein type: Ubiquitin conjugating system; Nuclear receptor co-regulator; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Transcription, coactivator/corepressor; Ligase

Chromosomal Location of Human Ortholog: 5q23.3-q31.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; androgen receptor binding; small conjugating protein ligase activity; zinc ion binding; transcription coactivator activity; ligase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; protein ubiquitination; signal transduction; response to estradiol stimulus