Western Blot (WB)
(Western Blot analysis of RNF111 in TM4 cell lysate using RNF111 Rabbit pAb at dilution 1/200)
Rabbit anti-Human, Mouse RNF111 Polyclonal Antibody | anti-RNF111 antibody
RNF111 Rabbit pAb
IHC-P: 1:50-1:300
Western Blot (WB)
(Western Blot analysis of RNF111 in TM4 cell lysate using RNF111 Rabbit pAb at dilution 1/200)
Western Blot (WB)
(Western Blot analysis of RNF111 in TM4 cell lysate using RNF111 Rabbit pAb at dilution 1/200)
Immunohistochemistry (IHC)
(Immunohistochemistry of RNF111 in paraffin-embedded Human gastric cancer tissue using RNF111 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)
Immunohistochemistry (IHC)
(Immunohistochemistry of RNF111 in paraffin-embedded Human gastric cancer tissue using RNF111 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
Uniprot Description
RNF111: Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin- protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase
Chromosomal Location of Human Ortholog: 15q21
Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; zinc ion binding; SUMO polymer binding; SMAD binding; ligase activity
Biological Process: transcription initiation from RNA polymerase II promoter; protein polyubiquitination; transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; gene expression; ubiquitin-dependent SMAD protein catabolic process; pattern specification process
Research Articles on RNF111
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Product Notes
The RNF111 rnf111 (Catalog #AAA4760332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF111 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNF111 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:50-1:300. Researchers should empirically determine the suitability of the RNF111 rnf111 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF111, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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