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Western Blot (WB) (Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse RIPK4 Polyclonal Antibody | anti-RIPK4 antibody

RIPK4, CT (Receptor-interacting Serine/Threonine-protein Kinase 4, Ankyrin Repeat Domain-containing Protein 3, PKC-delta-interacting Protein Kinase, ANKRD3, DIK) (FITC)

Gene Names
RIPK4; DIK; PKK; PPS2; RIP4; ANKK2; NKRD3; ANKRD3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
RIPK4; Polyclonal Antibody; CT (Receptor-interacting Serine/Threonine-protein Kinase 4; Ankyrin Repeat Domain-containing Protein 3; PKC-delta-interacting Protein Kinase; ANKRD3; DIK) (FITC); anti-RIPK4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ANKRD3. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-RIPK4 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human ANKRD3 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(ANKRD3 Antibody (Q817) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANKRD3 antibody detected the ANKRD3 protein (arrow).)

Western Blot (WB) (ANKRD3 Antibody (Q817) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANKRD3 antibody detected the ANKRD3 protein (arrow).)
Product Categories/Family for anti-RIPK4 antibody
References
1. Hattori, M., et al., Nature 405(6784):311-319 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,441 Da
NCBI Official Full Name
receptor-interacting serine/threonine-protein kinase 4
NCBI Official Synonym Full Names
receptor-interacting serine-threonine kinase 4
NCBI Official Symbol
RIPK4
NCBI Official Synonym Symbols
DIK; PKK; PPS2; RIP4; ANKK2; NKRD3; ANKRD3
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 4; protein kinase C-associated kinase; PKC-delta-interacting protein kinase; serine/threonine-protein kinase ANKRD3; ankyrin repeat domain-containing protein 3
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 4
UniProt Gene Name
RIPK4
UniProt Synonym Gene Names
ANKRD3; DIK
UniProt Entry Name
RIPK4_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that interacts with protein kinase C-delta. The encoded protein can also activate NFkappaB and is required for keratinocyte differentiation. This kinase undergoes autophosphorylation. [provided by RefSeq, Jul 2008]

Uniprot Description

ANKRD3: a TKL protein kinase of the RIPK family. Activates NFkappaB when overexpressed in cell lines and is required for keratinocyte differentiation in vivo. Appears to activate NFkappaB in both a kinase-dependent as well as a kinase-independent manner. Contains 10 ANK repeats. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, TKL; Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: morphogenesis of an epithelium; protein amino acid phosphorylation; activation of NF-kappaB transcription factor

Disease: Popliteal Pterygium Syndrome, Lethal Type

Research Articles on RIPK4

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Product Notes

The RIPK4 ripk4 (Catalog #AAA6497876) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RIPK4, CT (Receptor-interacting Serine/Threonine-protein Kinase 4, Ankyrin Repeat Domain-containing Protein 3, PKC-delta-interacting Protein Kinase, ANKRD3, DIK) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RIPK4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RIPK4 ripk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIPK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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