Rabbit RIP140 Polyclonal Antibody | anti-RIP140 antibody

RIP140 Antibody N-epitope

Cat. Num. AAA544709

• Gene Names: Nrip1; RIP140; AA959574; AW456757; 9630050P12; 6030458L20Rik; 8430438I05Rik
• Reactivity: Chicken, Human, Monkey, Rat
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: RIP140; Polyclonal Antibody; RIP140 Antibody N-epitope; NRIP 1; Nuclear factor RIP 140; Nuclear receptor interacting protein 1; Receptor-interacting protein 140; RIP140 antibody; anti-RIP140 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Monkey, Rat
• Clonality: Polyclonal
• Specificity: Recognizes RIP140 at the N terminal region containing the HDAC binding domain.
• Concentration: 0.60 ug/ul in antibody stabilization buffer (varies by lot)
• Sequence Length: 4307

• Applicable Applications for anti-RIP140 antibody: Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Confocal Microscopy||1:250; Dot Blot: 1:10,000; ELISA: 1:10,000; Immunocytochemistry: 1:250; Immunofluorescence: 1:250; Immunohistochemistry: 1:250; Immunoprecipitation: 1:200; Western Blot: 1:1,000
• Immunogen: Synthetic peptide taken within amino acid region 50-100 on mouse receptor interacting protein 140.
• Expression: Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.
• Molecular Function: Core promoter sequence-specific DNA binding; Estrogen Receptor binding; Glucocortioid receptor binding; Histone deacetylase binding; Nuclear hormone receptor binding; Receptor binding; Transcription coactivator/corepressor activity
• Structure: Contains at least 4 autonomous repression domains (RD1-4).By similarity; Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors.; The Ligand-dependent nuclear receptor binding region is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB.
• Subcellular Location: Nucleus
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for anti-RIP140 antibody

Receptor interacting protein 140 Antibody
Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.

NCBI and Uniprot Product Information

• NCBI GI #: 3820493
• NCBI GeneID: 268903
• UniProt Accession #: Q8CBD1; Q3U166; Q8C3Y8; Q9Z2K2
• Molecular Weight: 126,337 Da
• NCBI Official Full Name: Mus musculus receptor interacting protein 140 mRNA, complete cds
• NCBI Official Synonym Full Names: nuclear receptor interacting protein 1
• NCBI Official Symbol: Nrip1
• NCBI Official Synonym Symbols: RIP140; AA959574; AW456757; 9630050P12; 6030458L20Rik; 8430438I05Rik
• NCBI Protein Information: nuclear receptor-interacting protein 1
• UniProt Protein Name: Nuclear receptor-interacting protein 1
• UniProt Gene Name: Nrip1
• UniProt Entry Name: NRIP1_MOUSE

Uniprot Description

RIP140: Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH. Interacts with ZNF366.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: cell junction; histone deacetylase complex; nuclear chromatin; nuclear speck; nucleolus; nucleoplasm; nucleus

Molecular Function: estrogen receptor binding; glucocorticoid receptor binding; histone deacetylase binding; nuclear hormone receptor binding; protein binding; receptor binding; retinoic acid receptor binding; retinoid X receptor binding; transcription coactivator activity; transcription cofactor activity; transcription corepressor activity

Biological Process: circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle rupture; ovulation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; rhythmic process; sequestering of lipid; transcription, DNA-dependent