Rabbit anti-Human RING1 Polyclonal Antibody | anti-RING1 antibody

RING1, NT (RING1, RNF1, E3 ubiquitin-protein ligase RING1, Polycomb complex protein RING1, RING finger protein 1, Really interesting new gene 1 protein)

Cat. Num. AAA648390

• Gene Names: RING1; RNF1; RING1A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RING1; Polyclonal Antibody; NT (RING1; RNF1; E3 ubiquitin-protein ligase RING1; Polycomb complex protein RING1; RING finger protein 1; Really interesting new gene 1 protein); Anti -RING1; anti-RING1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RING1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: RING1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the N-terminal region of human RING1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RING1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RING1 antibody detected the RING1 protein (arrow).)

Related Product Information for anti-RING1 antibody

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.

Product Categories/Family for anti-RING1 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 296064
• NCBI GeneID: 6015
• UniProt Accession #: Q06587; Q5JP96; Q5SQW2; Q86V19; A8JZZ0
• Molecular Weight: 42,429 Da
• NCBI Official Full Name: RING1
• NCBI Official Synonym Full Names: ring finger protein 1
• NCBI Official Symbol: RING1
• NCBI Official Synonym Symbols: RNF1; RING1A
• NCBI Protein Information: E3 ubiquitin-protein ligase RING1; polycomb complex protein RING1; really interesting new gene 1 protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RING1
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RING1
• UniProt Synonym Gene Names: RNF1
• UniProt Entry Name: RING1_HUMAN

NCBI Description

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. Ref.12

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Component of chromatin-associated Polycomb (PcG) complexes. Interacts with BMI1

By similarity. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of a PRC1-like complex. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2. Interacts with PCGF2, RNF2; CBX6, CBX7 and CBX8. Interacts with PHC2

By similarity. Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.16 Ref.20

Subcellular location: Nucleus. Nucleus speckle Ref.20.

Miscellaneous: The hPRC-H complex purification reported by Ref.9 probably presents a mixture of different PRC1-like complexes.

Sequence similarities: Contains 1 RING-type zinc finger.