Rabbit anti-Human RIG-I Polyclonal Antibody | anti-RIG-I antibody

RIG-I, ID (Probable ATP-dependent RNA Helicase DDX58, DEAD-box Protein 58, Retinoic Acid-inducible Gene 1 Protein, RIG-1, Retinoic Acid-inducible Gene I Protein, DDX58) (AP)

Cat. Num. AAA6497851

• Gene Names: DDX58; RIGI; RIG-I; RLR-1; SGMRT2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RIG-I; Polyclonal Antibody; ID (Probable ATP-dependent RNA Helicase DDX58; DEAD-box Protein 58; Retinoic Acid-inducible Gene 1 Protein; RIG-1; Retinoic Acid-inducible Gene I Protein; DDX58) (AP); anti-RIG-I antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RIG-I.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-RIG-I antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human RIG-I (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX58 in 293 cell line lysates (35ug/lane). DDX58 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-RIG-I antibody

Antibodies; Enzymes

References

1. Li, K., et al., J. Biol. Chem. 280(17):16739-16747 (2005). 2. Breiman, A., et al., J. Virol. 79(7):3969-3978 (2005). 3. Cui, X.F., et al., Biochem. Cell Biol. 82(3):401-405 (2004). 4. Imaizumi, T., et al., Life Sci. 75(10):1171-1180 (2004). 5. Imaizumi, T., et al., Endothelium 11(3-4), 169-173 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 27881482
• NCBI GeneID: 23586
• NCBI Accession #: NP_055129
• NCBI GenBank Nucleotide #: NM_014314.3
• UniProt Accession #: Q96C10; Q5HYE1; Q5VYT1; Q9NT04; A2RU81; O95786
• Molecular Weight: 101,377 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX58
• NCBI Official Synonym Full Names: DExD/H-box helicase 58
• NCBI Official Symbol: DDX58
• NCBI Official Synonym Symbols: RIGI; RIG-I; RLR-1; SGMRT2
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX58
• UniProt Protein Name: Probable ATP-dependent RNA helicase DHX58
• UniProt Gene Name: DHX58
• UniProt Synonym Gene Names: D11LGP2E; LGP2; RLR-3; RLR
• UniProt Entry Name: DHX58_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily.

Protein type: Helicase; EC 3.6.4.13; Inhibitor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm

Molecular Function: protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: viral reproduction; negative regulation of innate immune response; metabolic process; response to virus; positive regulation of interferon type I production; innate immune response; defense response to virus; negative regulation of interferon type I production; regulation of innate immune response