Rabbit RICH2 Polyclonal Antibody | anti-ARHGAP44 antibody

RICH2 Antibody

Cat. Num. AAA9629130

• Gene Names: ARHGAP44; RICH2; NPC-A-10
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RICH2; Polyclonal Antibody; RICH2 Antibody; KIAA0672; NPC A 10; Rho GTPase activating protein RICH2; Rho type GTPase activating protein RICH2; RhoGAP interacting with CIP4 homologs protein 2; anti-ARHGAP44 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RICH2 Antibody detects endogenous levels of total RICH2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ARHGAP44 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Rho-GAP domain is required to promote GRIA1 exocytosis from recycling endosomes. Rho-GAP and BAR domains are necessary for the control of long-term potentiation in hippocampal neurons (By similarity). In dendrites, BAR domain mediates the recruitment to patches where plasma membrane is deformed by acto-myosin mediated contractile forces (By similarity).
• Subunit Structure: Interacts with BST2 (via cytoplasmic domain). Interacts (probably via PDZ-binding motif) with SHANK3 (via PDZ domain); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis.
• Tissue Specificity: Highly expressed in brain. Expressed at weak level in other tissues.
• Function: GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (By similarity). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (By similarity).
• Note: In CA1 hippocampal synapses, detected at both presynaptic and postsynaptic sites (By similarity). Located in convoluted dendritic plasma membrane sections enriched in polymerized actin and myosin (patches) along dendrites where often emerge filopodia (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ARHGAP44 antibody

Cell projection; Dendritic spine; Recycling endosome; Cell junction; Synapse; Presynapse; Dendrite; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 1007374019
• NCBI GeneID: 9912
• NCBI Accession #: NP_001308095.1
• NCBI GenBank Nucleotide #: NM_001321166.1
• UniProt Accession #: Q17R89; O75160; Q7Z5Z7; Q9Y4Q4; A6NCP5; A8MQB2
• Molecular Weight: 88,521 Da
• NCBI Official Full Name: rho GTPase-activating protein 44 isoform 3
• NCBI Official Synonym Full Names: Rho GTPase activating protein 44
• NCBI Official Symbol: ARHGAP44
• NCBI Official Synonym Symbols: RICH2; NPC-A-10
• NCBI Protein Information: rho GTPase-activating protein 44
• UniProt Protein Name: Rho GTPase-activating protein 44
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP44
• UniProt Synonym Gene Names: KIAA0672; RICH2; RICH-2

Uniprot Description

GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. May act as a GAP for CDC42 and RAC1. Endosomal recycling protein which, in association with SHANK3, is involved in synaptic plasticity. Promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.