Rabbit anti-Human, Mouse Ribophorin Polyclonal Antibody | anti-AT2G01720 antibody

Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1)

Cat. Num. AAA627995

• Gene Names: AT2G01720; T8O11.11; T8O11_11
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Ribophorin; Polyclonal Antibody; CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit; Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1; Ribophorin I; Ribophorin-1; RPN-I; RPN1); Anti -Ribophorin; anti-AT2G01720 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPN1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-AT2G01720 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human RPN1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-RPN1 Pab is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).)

Related Product Information for anti-AT2G01720 antibody

Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.

Product Categories/Family for anti-AT2G01720 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 15226341
• NCBI GeneID: 814701
• NCBI Accession #: NP_178281.1
• NCBI GenBank Nucleotide #: NM_126233.5
• UniProt Accession #: Q9ZUA0
• Molecular Weight: 52,219 Da
• NCBI Official Full Name: ribophorin I
• NCBI Official Symbol: AT2G01720
• NCBI Official Synonym Symbols: T8O11.11; T8O11_11
• NCBI Protein Information: ribophorin I
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B
• UniProt Gene Name: OST1B
• UniProt Synonym Gene Names: RPN1B; RPN-IB
• UniProt Entry Name: OST1B_ARATH

Uniprot Description

Function: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains

By similarity.

Catalytic activity: Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway: Protein modification; protein glycosylation.

Subunit structure: Component of the oligosaccharyltransferase (OST) complex

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity.

Sequence similarities: Belongs to the OST1 family.