Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Ribonuclease III, Nuclear (RNASEN) Polyclonal Antibody | anti-RNASEN antibody
Polyclonal Antibody to Ribonuclease III, Nuclear (RNASEN)
MGHHHHHHSG S- IGVIFTH VRLLARAFTL RTVGFNHLTL GHNQRMEFLG DSIMQLVATE YLFIHFPDHH EGHLTLLRSS LVNNRTQAKV AEELGMQEYA ITNDKTKRPV ALRTKTLADL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
Uniprot Description
DROSHA: a nuclear type-III RNase that, along with the DGCR8, executes the initial step of microRNA (miRNA) processing in the nucleus. Cleaves the local hairpin structures embedded in long primary microRNA transcripts (pri-miRNAs) into pre-miRNAs. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Interacts with Sp1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.26.3; Nucleolus; RNA processing; RNA-binding; Ribonuclease
Chromosomal Location of Human Ortholog: 5p13.3
Cellular Component: focal adhesion; nucleolus; nucleoplasm; nucleus; plasma membrane; postsynaptic density
Molecular Function: DEAD/H-box RNA helicase binding; double-stranded RNA binding; lipopolysaccharide binding; metal ion binding; protein binding; protein homodimerization activity; ribonuclease III activity; RNA binding; SMAD binding
Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; pre-microRNA processing; primary microRNA processing; regulation of inflammatory response; regulation of regulatory T cell differentiation; ribosome biogenesis; RNA interference, production of siRNA; rRNA catabolic process
Research Articles on RNASEN
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Product Notes
The RNASEN drosha (Catalog #AAA2017464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Ribonuclease III, Nuclear (RNASEN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease III, Nuclear (RNASEN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the RNASEN drosha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- IGVIFTH VRLLARAFTL RTVGFNHLTL GHNQRMEFLG DSIMQLVATE YLFIHFPDHH EGHLTLLRSS LVNNRTQAKV AEELGMQEYA ITNDKTKRPV ALRTKTLADL. It is sometimes possible for the material contained within the vial of "Ribonuclease III, Nuclear (RNASEN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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