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Western Blot (WB) (Western blot analysis of Rhox-10 in 12 day mouse testis in the 1) absence and 2) presence of immunizing peptide using MBS649974 at 1:2000. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Rabbit anti-Mouse Rhox-10 Polyclonal Antibody | anti-RHOXF1 antibody

Rhox-10 (Reproductive Homeobox on X Chromosome 10, Reproductive Homeobox (Rhox) 10)

Gene Names
RHOXF1; OTEX; PEPP1
Reactivity
Mouse
Applications
Western Blot
Purity
Serum
Serum.
Synonyms
Rhox-10; Polyclonal Antibody; Rhox-10 (Reproductive Homeobox on X Chromosome 10; Reproductive Homeobox (Rhox) 10); Anti -Rhox-10 (Reproductive Homeobox on X Chromosome 10; anti-RHOXF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Rhox-10. Species sequence homology: human, rat, monkey, equine, chimpanzee, bovine, chicken.
Purity/Purification
Serum
Serum.
Form/Format
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Applicable Applications for anti-RHOXF1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000-1:2000
Immunogen
Synthetic peptide corresponding to aa90-104 (NSKKYTNAQMCELEK) of mouse Rhox-10.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Rhox-10 in 12 day mouse testis in the 1) absence and 2) presence of immunizing peptide using MBS649974 at 1:2000. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Western Blot (WB) (Western blot analysis of Rhox-10 in 12 day mouse testis in the 1) absence and 2) presence of immunizing peptide using MBS649974 at 1:2000. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Related Product Information for anti-RHOXF1 antibody
Reproductive homeobox on X chromosome 10, also known as Reproductive homeobox 10, is a recently identified nuclear protein product of a 12 X-linked novel homeobox gene family that plays an important role during postnatal testis development. The members of this family are characterized by a conserved 60aa DNA-binding homeodomain. These transcription factors not only play an important role in various developmental processes such as DNA-dependent transcriptional regulation, body-plan specification, pattern formation, and cell fate determination, but also in understanding the development and physiology of the human reproductive system.
Product Categories/Family for anti-RHOXF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,542 Da
NCBI Official Full Name
rhox homeobox family member 1
NCBI Official Synonym Full Names
Rhox homeobox family, member 1
NCBI Official Symbol
RHOXF1
NCBI Official Synonym Symbols
OTEX; PEPP1
NCBI Protein Information
rhox homeobox family member 1; PEPP subfamily gene 1; paired-like homeobox protein PEPP-1; ovary-, testis- and epididymis-expressed gene protein
UniProt Protein Name
Rhox homeobox family member 1
Protein Family
UniProt Gene Name
RHOXF1
UniProt Synonym Gene Names
OTEX; PEPP1
UniProt Entry Name
RHXF1_HUMAN

NCBI Description

This gene is a member of the PEPP subfamily of paired-like homoebox genes. The gene may be regulated by androgens and epigenetic mechanisms. The encoded nuclear protein is likely a transcription factor that may play a role in human reproduction. [provided by RefSeq, Dec 2012]

Uniprot Description

Function: May be a transcription factor involved in reproductive processes.

Subunit structure: Does not interact with itself.

Subcellular location: Nucleus Ref.1.

Tissue specificity: Ovary, testis and epididymis. Also detected in the prostate and the mammary gland. Expressed in many tumor cell lines derived from acute lymphocytic leukemia, prostate, endometrial adenocarcinoma, melanoma, bladder carcinoma, colon carcinoma, erythroleukemia and breast carcinoma. Not expressed in placenta. Ref.4

Induction: By androgen.

Domain: Mutagenesis of amino acids 147 to 164 and 155 to 164 lead to a major cytoplasmic localization, with only minor localization in the nucleus.

Sequence similarities: Belongs to the paired-like homeobox family. PEPP subfamily.Contains 1 homeobox DNA-binding domain.

Sequence caution: The sequence AAC78617.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on RHOXF1

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Product Notes

The RHOXF1 rhoxf1 (Catalog #AAA649974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rhox-10 (Reproductive Homeobox on X Chromosome 10, Reproductive Homeobox (Rhox) 10) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rhox-10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000-1:2000. Researchers should empirically determine the suitability of the RHOXF1 rhoxf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rhox-10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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