Rabbit anti-Human, Mouse RHOU Polyclonal Antibody | anti-RHOU antibody

RHOU Antibody

Cat. Num. AAA9629999

• Gene Names: RHOU; ARHU; G28K; WRCH1; hG28K; CDC42L1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RHOU; Polyclonal Antibody; RHOU Antibody; ARHU; CDC42 L1; CDC42 like GTPase; CDC42-like GTPase 1; CDC42L1; G28K; GTP binding protein like 1; GTP binding protein SB128; GTP-binding protein-like 1; hG28K; mG28K; Ras homolog gene family member U; Ras homolog gene family member U isoform CRA a; Ras like gene family member U; Rho GTPase like protein ARHU; Rho GTPase-like protein ARHU; Rho related GTP binding protein RhoU; Rho-related GTP-binding protein RhoU; Rhou; RHOU_HUMAN; Ryu GTPase; Wnt-1 responsive Cdc42 homolog 1; Wnt1 responsive Cdc42 homolog; WRCH 1; WRCH-1; anti-RHOU antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RHOU Antibody detects endogenous levels of RHOU.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RHOU antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RHOU.
• Conjugation: Unconjugated
• Similarity: Belongs to the small GTPase superfamily. Rho family.
• Post Translational Modifications: Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.
• Subunit Structure: Interacts with PAK3. Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31. Interacts with PTK2B/PYK2.
• Tissue Specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.
• Function: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
• Note: Localizes to podosomes in SRC-transformed cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RHOU antibody

Cell membrane; Lipid-anchor; Cytoplasmic side; Golgi apparatus membrane; Lipid-anchor; Cell junction; Focal adhesion; Cell projection; Podosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 11034843
• NCBI GeneID: 58480
• NCBI Accession #: NP_067028.1
• NCBI GenBank Nucleotide #: NM_021205.5
• UniProt Accession #: Q7L0Q8; Q59FE9; Q8TDQ2; B1AKN1
• Molecular Weight: 22,164 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoU
• NCBI Official Synonym Full Names: ras homolog family member U
• NCBI Official Symbol: RHOU
• NCBI Official Synonym Symbols: ARHU; G28K; WRCH1; hG28K; CDC42L1
• NCBI Protein Information: rho-related GTP-binding protein RhoU; RHOU; Ryu GTPase; 2310026M05Rik; CDC42-like GTPase 1; GTP-binding protein SB128; GTP-binding protein like 1; rho GTPase-like protein ARHU; ras-like gene family member U; wnt-1 responsive Cdc42 homolog 1; ras homolog g
• UniProt Protein Name: Rho-related GTP-binding protein RhoU
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOU
• UniProt Synonym Gene Names: WRCH-1
• UniProt Entry Name: RHOU_HUMAN

NCBI Description

This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]

Uniprot Description

RHOU: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP- bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 1q42.11-q42.3

Cellular Component: Golgi membrane; cell projection; focal adhesion; plasma membrane; podosome; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; metal ion binding

Biological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; Rac protein signal transduction; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; G1/S transition of mitotic cell cycle