Human GLO1 ELISA Kit | GLO1 elisa kit

Human GLO1 ELISA Kit PicoKine

Cat. Num. AAA127961

• Gene Names: GLO1; GLYI; GLOD1; HEL-S-74
• Reactivity: Human
• Applications: ELISA
• Synonyms: GLO1; N/A; Human GLO1 ELISA Kit PicoKine; GLO1 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: A2-M184
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for GLO1 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
  O.D.	0.027	0.042	0.086	0.256	0.523	1.068	1.375	1.962

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	350	1269	4565	378	1366	4475
  Standard deviation	19.25	65.99	164.34	19.28	61.47	214.8
  CV(%)	5.5%	5.2%	3.6%	5.1%	4.5%	4.8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	350	365	370	368	363	18.53	5.1%
  Sample 2	1269	1305	1288	1283	1286	46.31	3.6%
  Sample 3	4565	4541	4720	4690	4629	282.37	6.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human GLO1 PicoKine ELISA Kit standard curve)

Related Product Information for GLO1 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for GLO1 elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 134039205
• NCBI GeneID: 2739
• UniProt Accession #: Q04760; P78375; Q59EL0; Q5TZW3; Q96FC0; Q96J41; B2R6P7; B4DDV0
• Molecular Weight: Calculated: 19kDa/20kDa; Observed: 21kDa
• NCBI Official Full Name: Lactoylglutathione lyase
• NCBI Official Synonym Full Names: glyoxalase I
• NCBI Official Symbol: GLO1
• NCBI Official Synonym Symbols: GLYI; GLOD1; HEL-S-74
• NCBI Protein Information: lactoylglutathione lyase; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; glyoxalase domain containing 1; epididymis secretory protein Li 74; S-D-lactoylglutathione methylglyoxal lyase
• UniProt Protein Name: Lactoylglutathione lyase
• UniProt Gene Name: GLO1
• UniProt Synonym Gene Names: Glx I
• UniProt Entry Name: LGUL_HUMAN