Rabbit anti-Human RHEB Polyclonal Antibody | anti-RHEB antibody

RHEB, CT (RHEB, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain) (MaxLight 490)

Cat. Num. AAA6341879

• Gene Names: RHEB; RHEB2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: RHEB; Polyclonal Antibody; CT (RHEB; RHEB2; GTP-binding protein Rheb; Ras homolog enriched in brain) (MaxLight 490); Ras homolog enriched in brain; anti-RHEB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-RHEB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: RHEB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from the C-terminal region of human RHEB.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RHEB Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RHEB antibody detected the RHEB protein (arrow).)

Immunohistochemistry (IHC)

(RHEB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHEB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RHEB antibody

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

Product Categories/Family for anti-RHEB antibody

Antibodies; Binding Proteins

References

Zheng, H., et al. Cancer Lett. 297(1):117-125(2010). Kim, H.W., et al. Mol. Cell. Biol. 30(22):5406-5420(2010). Karassek, S., et al. J. Biol. Chem. 285(44):33979-33991(2010). Wagner, R.J., et al. Am. J. Physiol., Cell Physiol. 299 (1), C119-C127 (2010): Lu, Z.H., et al. Cancer Res. 70(8):3287-3298(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5032041
• NCBI GeneID: 6009
• NCBI Accession #: NP_005605.1
• NCBI GenBank Nucleotide #: NM_005614.3
• UniProt Accession #: Q15382; Q53Y56; Q99444; B3KWN6; D3DX13
• Molecular Weight: 20,497 Da
• NCBI Official Full Name: GTP-binding protein Rheb
• NCBI Official Synonym Full Names: Ras homolog enriched in brain
• NCBI Official Symbol: RHEB
• NCBI Official Synonym Symbols: RHEB2
• NCBI Protein Information: GTP-binding protein Rheb; Ras homolog enriched in brain 2
• UniProt Protein Name: GTP-binding protein Rheb
• Protein Family: Rheb-like protein
• UniProt Gene Name: RHEB
• UniProt Synonym Gene Names: RHEB2
• UniProt Entry Name: RHEB_HUMAN

NCBI Description

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rheb

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway