Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS646556. This demonstrates that MBS646556 detected the RHBDF2 protein (arrow).)
Rabbit anti-Human RHBDF2 Polyclonal Antibody | anti-RHBDF2 antibody
RHBDF2, NT (RHBDF2, IRHOM2, RHBDL5, RHBDL6, Inactive rhomboid protein 2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6) (MaxLight 650)
Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS646556. This demonstrates that MBS646556 detected the RHBDF2 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS646556. This demonstrates that MBS646556 detected the RHBDF2 protein (arrow).)
Testing Data
(All lanes: MBS646556 (1:2000) Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 97kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes: MBS646556 (1:2000) Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 97kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry (FFPE) staining of RHBDF2 in human duodenum tissue usingMBS646556. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS646556 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry (FFPE) staining of RHBDF2 in human duodenum tissue usingMBS646556. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS646556 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry (FFPE) staining of RHBDF2 in human kidney tissue usingMBS646556. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS646556 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry (FFPE) staining of RHBDF2 in human kidney tissue usingMBS646556. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS646556 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration
By similarity.
Subunit structure: Interacts with EGF
By similarity.
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein
By similarity. Cell membrane Ref.7.
Tissue specificity: Found in the epidermis and esophageal epithelium. Ref.7
Involvement in disease: Tylosis with esophageal cancer (TOC) [MIM:148500]: An autosomal dominant syndrome characterized by diffuse palmoplantar keratoderma, oral leukokeratosis, and a high lifetime risk of esophageal cancer.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7
Sequence similarities: Belongs to the peptidase S54 family.
Sequence caution: The sequence AAH35829.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB15310.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Product Notes
The RHBDF2 rhbdf2 (Catalog #AAA6341837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHBDF2, NT (RHBDF2, IRHOM2, RHBDL5, RHBDL6, Inactive rhomboid protein 2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHBDF2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RHBDF2 rhbdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHBDF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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