Rabbit RGS8 Polyclonal Antibody | anti-RGS8 antibody

RGS8 Polyclonal Antibody

Cat. Num. AAA9129947

• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: RGS8; Polyclonal Antibody; RGS8 Polyclonal Antibody ; anti-RGS8 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 180

• Applicable Applications for anti-RGS8 antibody: Western Blot (WB)
• Application Notes: WB: 1:200 - 1:500
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180of human RGS8 (NP_001095920.1)
• Species Reactivity/Cross Reactivity: Human, Mouse, Rat
• Category: Primary Antibody
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RGS8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Related Product Information for anti-RGS8 antibody

Background: This gene is a member of the regulator of G protein signaling (RGS) family and encodesa protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins areregulatory and structural components of G protein-coupled receptor complexes. Theyaccelerate transit through the cycle of GTP binding and hydrolysis to GDP, therebyterminating signal transduction, but paradoxically, also accelerate receptor-stimulatedactivation

Product Categories/Family for anti-RGS8 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 156416024
• NCBI GeneID: 85397
• NCBI Accession #: NP_001095920.1
• NCBI GenBank Nucleotide #: NM_001102450.2
• UniProt Accession #: P57771; Q3SYD2; B4DGL9
• Molecular Weight: Observed MW: 17kDa / 20kDa; Calculated MW: 20kDa / 22kDa
• NCBI Official Full Name: regulator of G-protein signaling 8 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 8
• NCBI Official Symbol: RGS8
• NCBI Protein Information: regulator of G-protein signaling 8
• UniProt Protein Name: Regulator of G-protein signaling 8
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS8
• UniProt Synonym Gene Names: RGS8
• UniProt Entry Name: RGS8_HUMAN

NCBI Description

This gene is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates G protein-coupled receptor signaling cascades, including signaling via muscarinic acetylcholine receptor CHRM2 and dopamine receptor DRD2 (). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:18434541). Modulates the activity of potassium channels that are activated in response to DRD2 and CHRM2 signaling ().