Rabbit RGS7 Polyclonal Antibody | anti-RGS7 antibody

RGS7 Polyclonal Antibody

Cat. Num. AAA3011691

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RGS7; Polyclonal Antibody; RGS7 Polyclonal Antibody; Regulator of G-protein signaling 7; anti-RGS7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS7 polyclonal antibody detects endogenous levels of RGS7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 487

• Applicable Applications for anti-RGS7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human RGS7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RGS7 polyclonal antibody)

Related Product Information for anti-RGS7 antibody

Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors. G proteins comprise alpha, beta and gamma subunits, and following activation the alpha subunit binds GTP and dissociates from the betagamma complex. A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Galpha isoforms by increasing the rate at which they convert GTP to GDP. A subfamily of RGS proteins expressed in the central nervous system contain, in addition to the highly conserved RGS domain, a characteristic GGL domain, or G protein gamma subunit-like domain, which mediates binding to Gbeta 5 subunits. This subfamily, which includes RGS6, RGS7, RGS9 and RGS11, associates with Gbeta 5 to form active GAP complexes that are predominantly localized to the cytosol. RGS/beta5 complexes preferentially target Galphao subunit for hydrolysis and inhibit Gbeta1gamma2-mediated activation of phospholipase C.

NCBI and Uniprot Product Information

• NCBI GI #: 156627563
• NCBI GeneID: 6000
• NCBI Accession #: NP_002915.3
• NCBI GenBank Nucleotide #: NP_002915.3
• UniProt Accession #: P49802
• Molecular Weight: ~ 58kDa
• NCBI Official Full Name: regulator of G-protein signaling 7 isoform 1
• NCBI Official Synonym Full Names: regulator of G protein signaling 7
• NCBI Official Symbol: RGS7
• NCBI Protein Information: regulator of G-protein signaling 7
• UniProt Protein Name: Regulator of G-protein signaling 7
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS7
• UniProt Synonym Gene Names: RGS7
• UniProt Entry Name: RGS7_HUMAN

Uniprot Description

RGS7: inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/GNG5 dimer. May play a role in synaptic vesicle exocytosis. May play important role in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations. Heterodimer with GNG5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5 (By similarity). Interacts with 14-3-3 protein Tau and SNAP25BP. Four alternatively spliced isoforms have been described.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 1q43|1q23.1

Cellular Component: cytoplasm; plasma membrane; heterotrimeric G-protein complex; nucleus; cytosol

Molecular Function: signal transducer activity; G-protein beta-subunit binding; GTPase activator activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity