Rabbit anti-Human, Mouse RGS20 Polyclonal Antibody | anti-RGS20 antibody

RGS20 Antibody

Cat. Num. AAA9610162

• Gene Names: RGS20; RGSZ1; ZGAP1; gz-GAP; g(z)GAP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RGS20; Polyclonal Antibody; RGS20 Antibody; G(z)GAP; Gz selective GTPase activating protein; Gz-GAP; Gz-selective GTPase-activating protein; HGNC:14600; Regulator of G protein signaling 20; Regulator of G-protein signaling 20; Regulator of G-protein signaling Z1; Regulator of Gz selective protein signaling 1; Regulator of Gz-selective protein signaling 1; RGS20_HUMAN; RGSZ1; ZGAP1; anti-RGS20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS20 antibody detects endogenous levels of total RGS20
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 273

• Applicable Applications for anti-RGS20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human RGS20
• Subcellular Location: Membrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.
• Tissue Specificity: Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RGS20 using HT29 whole cell lysates)

Related Product Information for anti-RGS20 antibody

Function: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).
Subunit Structure: Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 (By similarity).
Post-translational Modifications: Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity). N-and O-glycosylated in synapsomal membranes. Serine phosphorylated in synapsomal membranes. Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 557786129
• NCBI GeneID: 8601
• NCBI Accession #: NP_001273602.1
• NCBI GenBank Nucleotide #: NM_001286673.1
• UniProt Accession #: O76081; Q96BG9
• Molecular Weight: Observed: 44 kDa; Predicted: 44 kDa
• NCBI Official Full Name: regulator of G-protein signaling 20 isoform c
• NCBI Official Synonym Full Names: regulator of G protein signaling 20
• NCBI Official Symbol: RGS20
• NCBI Official Synonym Symbols: RGSZ1; ZGAP1; gz-GAP; g(z)GAP
• NCBI Protein Information: regulator of G-protein signaling 20
• UniProt Protein Name: Regulator of G-protein signaling 20
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS20
• UniProt Synonym Gene Names: RGSZ1; ZGAP1; RGS20; G(z)GAP; Gz-GAP

NCBI Description

The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins ().