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Western Blot (WB) (Western blot analysis of RGR Antibody (Center) in HL-60 cell line lysates (35ug/lane). RGR (arrow) was detected using the purified Pab.)

Rabbit anti-Human RGR Polyclonal Antibody | anti-RGR antibody

RGR, ID (RGR, RPE-retinal G protein-coupled receptor) (Azide free) (HRP)

Gene Names
RGR; RP44
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RGR; Polyclonal Antibody; ID (RGR; RPE-retinal G protein-coupled receptor) (Azide free) (HRP); RPE-retinal G protein-coupled receptor; anti-RGR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-RGR antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262~291 amino acids from the Central region of human RGR.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RGR Antibody (Center) in HL-60 cell line lysates (35ug/lane). RGR (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RGR Antibody (Center) in HL-60 cell line lysates (35ug/lane). RGR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RGR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RGR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RGR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RGR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RGR Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (RGR Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RGR antibody
This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively).
Product Categories/Family for anti-RGR antibody
References
Kochounian, H., et al. Exp. Eye Res. 88(6):1129-1136(2009). Radu, R.A., et al. J. Biol. Chem. 283(28):19730-19738(2008). Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008). Lin, M.Y., et al. Mol. Vis. 13, 1203-1214 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
291
NCBI Official Full Name
RPE-retinal G protein-coupled receptor
NCBI Official Synonym Full Names
retinal G protein coupled receptor
NCBI Official Symbol
RGR
NCBI Official Synonym Symbols
RP44
NCBI Protein Information
RPE-retinal G protein-coupled receptor; RGR-opsin; RPE retinal G-protein coupled receptor
UniProt Protein Name
RPE-retinal G protein-coupled receptor
UniProt Gene Name
RGR
UniProt Entry Name
RGR_HUMAN

NCBI Description

This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

RGR: Receptor for all-trans- and 11-cis-retinal. Binds preferentially to the former and may catalyze the isomerization of the chromophore by a retinochrome-like mechanism. Defects in RGR are the cause of retinitis pigmentosa type 44 (RP44). RP44 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; photoreceptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; visual perception; phototransduction; chemotaxis; protein-chromophore linkage

Disease: Retinitis Pigmentosa 44

Research Articles on RGR

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Product Notes

The RGR rgr (Catalog #AAA6341668) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RGR, ID (RGR, RPE-retinal G protein-coupled receptor) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RGR can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RGR rgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RGR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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