Rabbit anti-Human RFXAP Polyclonal Antibody | anti-RFXAP antibody

RFXAP, CT (RFXAP, Regulatory factor X-associated protein, RFX DNA-binding complex 36kD subunit) (Biotin)

Cat. Num. AAA6341578

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RFXAP; Polyclonal Antibody; CT (RFXAP; Regulatory factor X-associated protein; RFX DNA-binding complex 36kD subunit) (Biotin); RFX DNA-binding complex 36kD subunit; anti-RFXAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RFXAP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RFXAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human RFXAP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RFXAP Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RFXAP antibody detected the RFXAP protein (arrow).)

Related Product Information for anti-RFXAP antibody

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene.

Product Categories/Family for anti-RFXAP antibody

Antibodies; Binding Proteins

References

Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010). Bushway, M., et al. Immunogenetics 62(2):109-116(2010). Briggs, L., et al. Proteins 76(3):655-664(2009). Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008). van Eggermond, M.C., et al. Mol. Immunol. 45(10):2920-2928(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 187761332
• NCBI GeneID: 5994
• NCBI Accession #: NP_000529.1
• NCBI GenBank Nucleotide #: NM_000538
• UniProt Accession #: O00287; Q5VZM6; Q8TC40; B2R9T8
• Molecular Weight: 28,232 Da
• NCBI Official Full Name: Homo sapiens regulatory factor X-associated protein (RFXAP), mRNA
• NCBI Official Synonym Full Names: regulatory factor X-associated protein
• NCBI Official Symbol: RFXAP
• NCBI Protein Information: regulatory factor X-associated protein; RFX DNA-binding complex 36 kDa subunit; RFX-associated protein
• UniProt Protein Name: Regulatory factor X-associated protein
• Protein Family: Regulatory factor X-associated protein
• UniProt Gene Name: RFXAP
• UniProt Entry Name: RFXAP_HUMAN

NCBI Description

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RFXAP: Part of the RFX complex that binds to the X-box of MHC II promoters. Defects in RFXAP are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: nucleus

Molecular Function: DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent

Disease: Bare Lymphocyte Syndrome, Type Ii