Western Blot (WB)
(Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody)
Rabbit anti-Human, Mouse RFX3 Polyclonal Antibody | anti-RFX3 antibody
RFX3 Antibody
IHC: 1:100-1:300
ELISA: 1:40000
Western Blot (WB)
(Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
RFX3: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. Belongs to the RFX family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; DNA-binding
Chromosomal Location of Human Ortholog: 9p24.2
Cellular Component: transcription factor complex; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cell maturation; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; endocrine pancreas development
Research Articles on RFX3
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Product Notes
The RFX3 rfx3 (Catalog #AAA7116956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RFX3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RFX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 ELISA: 1:40000. Researchers should empirically determine the suitability of the RFX3 rfx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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