Rabbit anti-Human RFC2 Polyclonal Antibody | anti-RFC2 antibody

RFC2, NT (Replication Factor C Subunit 2, Activator 1 Subunit 2, Replication Factor C 40kD Subunit, RF-C 40kD Subunit, RFC40, Activator 1 40kD Subunit, A1 40kD Subunit) (MaxLight 650)

Cat. Num. AAA6497440

• Gene Names: RFC2; RFC40
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RFC2; Polyclonal Antibody; NT (Replication Factor C Subunit 2; Activator 1 Subunit 2; Replication Factor C 40kD Subunit; RF-C 40kD Subunit; RFC40; Activator 1 40kD Subunit; A1 40kD Subunit) (MaxLight 650); anti-RFC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFC2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-RFC2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RFC2 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol))

Western Blot (WB)

(Western blot analysis of anti-RFC2 Antibody (N-term) in K562 cell line lysates (35ug/lane). RFC2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(RFC2 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RFC2 antibody

Antibodies; Transcription Factors

References

1. Tomida, J., J. Biol. Chem. 283(14), 9071-9079 (2008). 2. Gupte, R.S., Cell Cycle 4(2), 323-329 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 31563534
• NCBI GeneID: 5982
• NCBI Accession #: NP_852136
• NCBI GenBank Nucleotide #: NM_181471
• UniProt Accession #: P35250; P32846; Q9BU93; B5BU07; D3DXG3
• Molecular Weight: 35,244 Da
• NCBI Official Full Name: replication factor C subunit 2 isoform 1
• NCBI Official Synonym Full Names: replication factor C (activator 1) 2, 40kDa
• NCBI Official Symbol: RFC2
• NCBI Official Synonym Symbols: RFC40
• NCBI Protein Information: replication factor C subunit 2; A1 40 kDa subunit; RF-C 40 kDa subunit; activator 1 subunit 2
• UniProt Protein Name: Replication factor C subunit 2
• Protein Family: Replication factor
• UniProt Gene Name: RFC2
• UniProt Synonym Gene Names: A1 40 kDa subunit; RF-C 40 kDa subunit; RFC40
• UniProt Entry Name: RFC2_HUMAN

Uniprot Description

RFC2: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; Golgi apparatus; DNA replication factor C complex; nucleus

Molecular Function: protein binding; ATP binding; DNA clamp loader activity

Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; DNA strand elongation during DNA replication; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; DNA repair; DNA replication; telomere maintenance