Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using Retinoid X Receptor beta Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Retinoid X Receptor beta Polyclonal Antibody | anti-RXRB antibody
Retinoid X Receptor beta Antibody
Gene Names
RXRB; NR2B2; DAUDI6; RCoR-1; H-2RIIBP
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
Peptide affinity purification
Synonyms
Retinoid X Receptor beta; Polyclonal Antibody; Retinoid X Receptor beta Antibody; DAUD I6; DAUDI6; H 2RIIBP; H2RIIBP; MGC1831; MHC class I promoter binding protein; NR2B2; nuclear receptor subfamily 2 group B member 2; RCoR 1; RCoR1; Retinoic acid receptor RXR beta; RXR beta; RXRB; RXRbeta; anti-RXRB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Retinoid X Receptor beta Antibody detects endogenous levels of total Retinoid X Receptor beta.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
533
Applicable Applications for anti-RXRB antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
The optimal dilutions should be determined by the end user.
IMPORTANT: for western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween® at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
The optimal dilutions should be determined by the end user.
IMPORTANT: for western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween® at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human Retinoid X Receptor beta, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using Retinoid X Receptor beta Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using Retinoid X Receptor beta Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612024 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612024 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Related Product Information for anti-RXRB antibody
Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE).
Subcellular Location: Nucleus. Cytoplasm.
Tissue Specificity: Expressed in aortic endothelial cells (at protein level) (PubMed:28167758). Expressed in monocytes (PubMed:26463675). Expressed in a variety of tumor cell lines.
Subunit Structure: Homodimer (in vitro) (PubMed:11782480). Heterodimer with other retinoic acid receptor family members. Binds DNA preferentially as a RAR/RXR heterodimer (PubMed:1310259). Interacts with NR1H3 (PubMed:12970175). Interacts with AKAP13 (By similarity).
Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.Belongs to the nuclear hormone receptor family. NR2 subfamily.
Subcellular Location: Nucleus. Cytoplasm.
Tissue Specificity: Expressed in aortic endothelial cells (at protein level) (PubMed:28167758). Expressed in monocytes (PubMed:26463675). Expressed in a variety of tumor cell lines.
Subunit Structure: Homodimer (in vitro) (PubMed:11782480). Heterodimer with other retinoic acid receptor family members. Binds DNA preferentially as a RAR/RXR heterodimer (PubMed:1310259). Interacts with NR1H3 (PubMed:12970175). Interacts with AKAP13 (By similarity).
Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.Belongs to the nuclear hormone receptor family. NR2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56kDa.
NCBI Official Full Name
retinoic acid receptor RXR-beta isoform 2
NCBI Official Synonym Full Names
retinoid X receptor beta
NCBI Official Symbol
RXRB
NCBI Official Synonym Symbols
NR2B2; DAUDI6; RCoR-1; H-2RIIBP
NCBI Protein Information
retinoic acid receptor RXR-beta
UniProt Protein Name
Retinoic acid receptor RXR-beta
Protein Family
UniProt Gene Name
RXRB
UniProt Synonym Gene Names
NR2B2
UniProt Entry Name
RXRB_HUMAN
NCBI Description
This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). The encoded protein forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Research Articles on RXRB
Similar Products
Product Notes
The RXRB rxrb (Catalog #AAA9612024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Retinoid X Receptor beta Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Retinoid X Receptor beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000 The optimal dilutions should be determined by the end user. IMPORTANT: for western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween® at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the RXRB rxrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Retinoid X Receptor beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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