anti-Mouse Resistin-like Molecule, beta Polyclonal Antibody | anti-RETNLB antibody
Anti - Resistin-like Molecule, beta (RELMb)
Gene Names
RETNLB; XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified over the control-peptide Sepharose
Synonyms
Resistin-like Molecule; beta; Polyclonal Antibody; Anti - Resistin-like Molecule; beta (RELMb); anti-RETNLB antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse sequence is only 42% conserved in human RELM-beta. We recommend the use of antibody that is raised to human RELM-beta sequence. No significant sequence was found with resistin or RELM-alpha. Antibody crossreactivity in various species is not established. We recommend the use of control peptide (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity. Control peptides, because of their small size (< 2kD), are not suitable for Western.
Purity/Purification
Affinity purified over the control-peptide Sepharose
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Sequence Length
111
Applicable Applications for anti-RETNLB antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique. Resistin is ~9kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique. Resistin is ~9kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
A 14-aa peptide sequence near the N-terminus of Mouse RELM-beta (1) was coupled with KLH (carrier protein)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-RETNLB antibody
References
• :1. Steppan CM et al (2001) Nature 409, 307; Steppan CM et al (2001) PNAS 98, 502; Holcomb IN et al (2000) EMBO J. 19, 4046-4055; Kim K-H et al (2001) J. Biol. Chem. 276, 11252-11256 We recommend the use of control peptide (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity. Click here for a general protocol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,747 Da
NCBI Official Full Name
resistin-like beta
NCBI Official Synonym Full Names
resistin like beta
NCBI Official Symbol
RETNLB
NCBI Official Synonym Symbols
XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
NCBI Protein Information
resistin-like beta; OTTHUMP00000215152; found in inflammatory zone 1; colon carcinoma-related gene protein; cysteine-rich secreted protein FIZZ2; cysteine-rich secreted A12-alpha-like protein 1; cysteine-rich secreted protein A12-alpha-like 1; colon and small intestine-specific cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2
UniProt Protein Name
RELM-beta protein
Protein Family
UniProt Gene Name
RELM-beta
UniProt Entry Name
Q2UXL7_HUMAN
Similar Products
Product Notes
The RETNLB relm-beta (Catalog #AAA611595) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Resistin-like Molecule, beta (RELMb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Resistin-like Molecule, beta can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique. Resistin is ~9kD. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the RETNLB relm-beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Resistin-like Molecule, beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
