Rabbit REPS1 Polyclonal Antibody | anti-REPS1 antibody

REPS1 Antibody

Cat. Num. AAA9611219

• Gene Names: REPS1; NBIA7; RALBP1
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: REPS1; Polyclonal Antibody; REPS1 Antibody; RALBP1; RalBP1 associated Eps domain containing protein 1; RalBP1 interacting protein 1; RalBP1-associated Eps domain-containing protein 1; RalBP1-interacting protein 1; REPS1_HUMAN; anti-REPS1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: REPS1 Antibody detects endogenous levels of REPS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 769

• Applicable Applications for anti-REPS1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human REPS1.
• Tissue Specificity: Widely expressed with highest levels in heart and testis.
• Subcellular Location: Membrane > Clathrin-Coated Pit. Colocalize with ITSN1 at the plasma membrane in structures that are most probably Clathrin-Coated Pits.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of Rat Heart tissue sample,using REPS1 Antibody.)

Related Product Information for anti-REPS1 antibody

Description: May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.

Post Translational Modifications: EGF stimulates phosphorylation on Tyr-residues.

Subunit Structure: Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase (By similarity). Interacts with AMPH, ITSN1 (via SH3 domains) and SGIP1; may be involved in clathrin-mediated endocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 190886437
• NCBI GeneID: 85021
• NCBI Accession #: NP_001122089.1
• NCBI GenBank Nucleotide #: NP_001122089.1
• UniProt Accession #: Q96D71
• Molecular Weight: Observed: 80KD; Predicted: 87 kDa
• NCBI Official Full Name: ralBP1-associated Eps domain-containing protein 1 isoform b
• NCBI Official Synonym Full Names: RALBP1 associated Eps domain containing 1
• NCBI Official Symbol: REPS1
• NCBI Official Synonym Symbols: NBIA7; RALBP1
• NCBI Protein Information: ralBP1-associated Eps domain-containing protein 1
• UniProt Protein Name: RalBP1-associated Eps domain-containing protein 1
• Protein Family: RalBP1-associated Eps domain-containing protein
• UniProt Gene Name: REPS1
• UniProt Entry Name: REPS1_HUMAN

NCBI Description

This gene encodes a signaling adaptor protein with two EH domains that interacts with proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

Uniprot Description

REPS1: a vesicle-associated protein that influences endocytosis through its ability to interact with Rab11FIP2 and RALBP1. An EGFR substrate that interacts with CRK and GRB2. May coordinate the cellular actions of activated EGF receptors and Ral-GTPases. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2. Three alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 6q24.1

Cellular Component: plasma membrane; coated pit

Molecular Function: protein binding; calcium ion binding; SH3 domain binding

Biological Process: receptor-mediated endocytosis