Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
Rabbit anti-Human RENT1 Polyclonal Antibody | anti-UPF1 antibody
RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae)
Gene Names
UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1; FLJ43809; FLJ46894; KIAA0221
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
RENT1; Polyclonal Antibody; RENT1 (Regulator of Nonsense Transcripts 1; ATP-dependent Helicase RENT1; Delta Helicase; FLJ43809; FLJ46894; hUPF1; KIAA0221; Nonsense mRNA Reducing Factor 1; NORF1; pNORF1; pNORF-1; UP Frameshift 1; UPF1; Up Frameshift Mutation 1 Homolog (S. cerevisiae); Anti -RENT1 (Regulator of Nonsense Transcripts 1; anti-UPF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human RENT1. Species sequence homology: mouse, rat, bovine, canine.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-UPF1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.5-2ug/ml
Dilution: Western Blot: 0.5-2ug/ml
Immunogen
Synthetic linear peptide corresponding to RENT1.
Positive Control
U2OS cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
Related Product Information for anti-UPF1 antibody
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. RENT1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
Product Categories/Family for anti-UPF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,531 Da
NCBI Official Full Name
regulator of nonsense transcripts 1
NCBI Official Synonym Full Names
UPF1 regulator of nonsense transcripts homolog (yeast)
NCBI Official Symbol
UPF1
NCBI Official Synonym Symbols
HUPF1; NORF1; RENT1; smg-2; pNORF1; FLJ43809; FLJ46894; KIAA0221
NCBI Protein Information
regulator of nonsense transcripts 1; delta helicase; UP Frameshift 1; yeast Upf1p homolog; ATP-dependent helicase RENT1; nonsense mRNA reducing factor 1; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; smg-2 homolog, nonsense mediated mRNA decay factor
UniProt Protein Name
PNORF1
Protein Family
UniProt Gene Name
pNORF1
UniProt Entry Name
O60385_HUMAN
NCBI Description
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]
Research Articles on UPF1
Similar Products
Product Notes
The UPF1 pnorf1 (Catalog #AAA620548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RENT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.5-2ug/ml. Researchers should empirically determine the suitability of the UPF1 pnorf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RENT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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