Rabbit REF6 Polyclonal Antibody | anti-REF6 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) REF6 Polyclonal Antibody

• Gene Names: REF6; JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: REF6; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) REF6 Polyclonal Antibody; REF6 Antibody; Lysine-specific demethylase REF6; EC 1.14.11.-; Jumonji domain-containing protein 12; Lysine-specific histone demethylase REF6; Protein RELATIVE OF EARLY FLOWERING 6; REF6 JMJ12 PKDM9A At3g48430 T29H11_50; anti-REF6 antibody

• Host: Rabbit
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-REF6 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Arabidopsis thaliana REF6 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 79586698
• NCBI GeneID: 824002
• NCBI Accession #: NP_680116.2
• NCBI GenBank Nucleotide #: NM_148863.4
• UniProt Accession #: Q9STM3
• Molecular Weight: 152,629 Da
• NCBI Official Full Name: relative of early flowering 6
• NCBI Official Symbol: REF6
• NCBI Official Synonym Symbols: JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
• NCBI Protein Information: relative of early flowering 6
• UniProt Protein Name: Lysine-specific demethylase REF6
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: REF6

NCBI Description

Relative of Early Flowering 6 (REF6) encodes a Jumonji N/C and zinc finger domain-containing protein that acts as a positive regulator of flowering in an FLC-dependent pathway. REF6 mutants have hyperacetylation of histone H4 at the FLC locus. REF6 interacts with BES1 in a Y2H assay and in vitro. REF6 may play a role in brassinoteroid signaling by affecting histone methylation in the promoters of BR-responsive genes. It is most closely related to the JHDM3 subfamily of JmjN/C proteins.

Uniprot Description

Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me (PubMed:21642989, PubMed:27111035). Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay (PubMed:20711170). Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli (PubMed:18467490). Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation (PubMed:27111034, PubMed:27111035). Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression (PubMed:15377760). Interacts with the NF-Y complexe to regulate SOC1 (PubMed:25105952). Mediates the recruitment of BRM to its target loci (PubMed:27111034).