Chicken Red Fluorescent Protein Polyclonal Antibody | anti-RFP antibody

Red Fluorescent Protein (RFP) (Biotin)

Cat. Num. AAA6497153

• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Red Fluorescent Protein; Polyclonal Antibody; Red Fluorescent Protein (RFP) (Biotin); anti-RFP antibody

• Host: Chicken
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes red fluorescent protein (RFP). Shows no reactivity to GFP.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RFP antibody: Western Blot (WB)
• Application Notes: WB: 1:10,000-1:30,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB.; Applications are based on unconjugated antibody.
• Immunogen: Recombinant red fluorescent protein (RFP), highly purified by affinity chromatography on Nickel column.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

SDS-Page

(Figure A. Purified recombinant GFP and RFP resolved by SDS-PAGE and stained with Coomassie blue. Lanes 1 and 2: 50 and 25 ng, respectively, of purified RFP; lane 3: 50 ng of purified GFP. Figure B. Immunoblot. Lanes 1 and 2: 12.5 and 4 ng of RFP; lane 3: 50 ng of GFP. Some cross reactivity of the RFP with GFP is observed due to the presence of the 6-His tag on GFP. RFP was used at 1:30,000 dilution and the HRP-conjugated rabbit anti-chicken antibody was used at 1:10,000. Bands were visualized using ECL.)

Product Categories/Family for anti-RFP antibody

Antibodies; Epitope Tag; RFP

NCBI and Uniprot Product Information

• NCBI GI #: 55976617
• UniProt Accession #: Q9U6Y8
• Molecular Weight: 25,931 Da
• NCBI Official Full Name: Red fluorescent protein drFP583
• UniProt Protein Name: Red fluorescent protein drFP583
• Protein Family: Red fluorescent protein
• UniProt Gene Name: DsRed
• UniProt Synonym Gene Names: DsRed
• UniProt Entry Name: RFP_DISSP

Uniprot Description

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.