Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30034'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.82 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30034' and pd.language_id = 1
Query
Database
1.76 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30034'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
2.38 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '30034'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '30034' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '30034'
⇄⧉testing_protocols => string (1166) "ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The ...
$value['testing_protocols']
ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC.jpg
⇄⧉products_description => string (695) "CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed...
$value['products_description']
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
⇄⧉testing_protocols => string (1166) "ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The ...
$value->a['testing_protocols']
ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC.jpg
⇄⧉products_description => string (695) "CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed...
$value->a['products_description']
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
⇄⧉testing_protocols => string (1166) "ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The ...
$value->d['testing_protocols']
ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC.jpg
⇄⧉products_description => string (695) "CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed...
$value->d['products_description']
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
⇄⧉products_description => string (383) "Possible adhesion molecule with a role in early hematopoiesis by mediating t...
$value[0]['_source']['products_description']
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
⇄⧉testing_protocols => string (871) "WB (Western Blot)||Western blot analysis of extracts of various cell lines, ...
$value[1]['_source']['testing_protocols']
WB (Western Blot)||Western blot analysis of extracts of various cell lines, using CD34 at 1:1000 dilution.||AAA30620_WB6.jpg!!IF (Immunofluorescence)||Immunofluorescence analysis of human placenta using CD34 at dilution of 1:100. Blue: DAPI for nuclear staining.||AAA30620_IF5.jpg!!IF (Immunofluorescence)||Immunofluorescence analysis of rat brain using CD34 at dilution of 1:100. Blue: DAPI for nuclear staining.||AAA30620_IF4.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of paraffin-embedded rat kidney using CD34 at dilution of 1:100 (40x lens).||AAA30620_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of paraffin-embedded mouse kidney using CD34 at dilution of 1:100 (40x lens).||AAA30620_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of paraffin-embedded human placenta using CD34 at dilution of 1:100 (40x lens).||AAA30620_IHC.jpg
⇄etc_term1 => string (62) "Immunogen||A synthetic peptide of human CD34 (NP_001020280.1)."
⇄⧉products_description => string (321) "The protein encoded by this gene may play a role in the attachment of stem c...
$value[1]['_source']['products_description']
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
⇄⧉testing_protocols => string (1166) "ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The ...
$value[2]['_source']['testing_protocols']
ICC (Immunocytochemistry)||ICC staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30034_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC2.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.||AAA30034_IHC.jpg
⇄⧉products_description => string (695) "CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed...
$value[2]['_source']['products_description']
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
⇄⧉search_terms => string (946) "aaa30034 rabbit human mouse rat dog monoclonal si16 01 proa affinity purifie...
$value[2]['_source']['search_terms']
aaa30034 rabbit human mouse rat dog monoclonal si16 01 proa affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunofluorescence if immunohistochemistry ihc immunoprecipitation ip 1:1000 1:2000 1:50 1:200 immunohistochemical analysis of paraffin embedded tonsil tissue using anti cd34 antibody counter stained with hematoxylin aaa30034_ihc kidney aaa30034_ihc2 aaa30034_ihc3 staining in a549 cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30034_icc4 huvec aaa30034_icc5 sh sy 5y aaa30034_icc6 antigen molecule cd34_human cluster designation 34 hematopoietic progenitor cell hpca1 mucosialin otthump00000034733 otthump00000034734 40,716 da 264769 aab25223.1 p28906 q15970 q15971 q5jta3 q5jta4 q9ujb1 a8k664 142230 total protein ab type recombinant immunogen conjugation unconjugated si1601 ph7.41 bsa40 designation34
⇄reactivity => string (29) "Human, Rat. Others not known."
$value[3]['_source']['reactivity']
⇄⧉specificity => string (745) "This antibody recognizes a transmembrane, heavily glycosylated protein of 90...
$value[3]['_source']['specificity']
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
⇄⧉purity => string (91) "Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Az...
$value[3]['_source']['purity']
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
⇄⧉form => string (151) "200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS wi...
$value[3]['_source']['form']
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄⧉storage_stability => string (175) "Antibody with azide - store at 2 to 8 degree C.<br>Antibody without azide - ...
$value[3]['_source']['storage_stability']
Antibody with azide - store at 2 to 8 degree C.<br>Antibody without azide - store at -20 to -80 degree C.<br>Antibody is stable for 24 months. Non-hazardous. No MSDS required.
⇄⧉app_notes => string (220) "Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of for...
$value[3]['_source']['app_notes']
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
⇄⧉search_terms => string (1407) "aaa23910 rabbit human rat others not known monoclonal igg hpca1 2598r purifi...
$value[3]['_source']['search_terms']
aaa23910 rabbit human rat others not known monoclonal igg hpca1 2598r purified ab with bsa and azide at 200ug ml or without 1.0mg of recombinant mab by protein a g prepared in 10mm pbs 0.05 also available this antibody recognizes transmembrane heavily glycosylated 90 120kda which is identified as cd34 its expression hallmark for identifying pluripotent hematopoietic stem progenitor cells gradually lost lineage committed progenitors differentiate marker choice staining blasts acute myeloid leukemia addition it expressed soft tissue tumors such solitary fibrous tumor gastrointestinal stromal found vascular endothelium additionally proliferating endothelial overexpress molecule than the non anti labels > 85 angiosarcoma kaposi s sarcoma but shows low specificity immunohistology formalin fixed 1 2ug 30 min rt tissues requires boiling sections tris 1mm edta ph 9.0 10 20 followed cooling minutes immunohistochemistry ihc paraffin embedded stained aaa23910_ihc colon carcinoma aaa23910_ihc2 renal cell aaa23910_ihc3 breast aaa23910_ihc4 tonsil aaa23910_ihc5 sds page analysis confirmation purity integrity aaa23910_sds6 antigen mucosialin isoform 110kda cd_antigen 68342038 np_001020280.1 p28906 nm_001025109.1 q15970 q15971 q5jta3 q5jta4 q9ujb1 a8k664 immunogen full length positive control kg cellular localization surface cytoplasmic chromosome location 1q32.2 glycosylated90 >85 fixed1 2ug30 ph9.0
⇄⧉products_description => string (677) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[4]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human VEGF-B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄products_references => string (3) "N/A"
$value[4]['_source']['products_references']
⇄⧉products_related_diseases => string (185) "Neoplasms||32!!Heart Diseases||11!!Nervous System Diseases||7!!Hypertrophy||...
$value[4]['_source']['products_related_diseases']
Neoplasms||32!!Heart Diseases||11!!Nervous System Diseases||7!!Hypertrophy||6!!Edema||5!!Liver Neoplasms||5!!Liver Diseases||5!!Drug Toxicity||5!!Hypertension||5!!Myocardial Ischemia||5
⇄⧉search_terms => string (444) "aaa13026 human no cross reaction with other factors typical testing data sta...
$value[4]['_source']['search_terms']
aaa13026 human no cross reaction with other factors typical testing data standard curve for reference only aaa13026_sc elisa kit vascular endothelial growth factor b vegf isoform vegfb 186 vrf vegfl related 21,261 da vegfb_human 4507887 np_003368.1 p49765 nm_003377.4 q16528 601398 samples serum plasma or cell culture supernatant detection range 1000 pg ml 15.6 sensitivity 5 intra assay precision ? 8 inter 12 vegfb186 sensitivity5 ?8 inter12
⇄⧉products_description => string (826) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[5]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat VEGF-B monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄products_references => string (3) "N/A"
$value[5]['_source']['products_references']
⇄⧉products_related_diseases => string (185) "Neoplasms||32!!Heart Diseases||11!!Nervous System Diseases||7!!Hypertrophy||...
$value[5]['_source']['products_related_diseases']
Neoplasms||32!!Heart Diseases||11!!Nervous System Diseases||7!!Hypertrophy||6!!Edema||5!!Liver Neoplasms||5!!Liver Diseases||5!!Drug Toxicity||5!!Hypertension||5!!Myocardial Ischemia||5
⇄⧉search_terms => string (388) "aaa13144 rat no cross reaction with other factors typical standard curve tes...
$value[5]['_source']['search_terms']
aaa13144 rat no cross reaction with other factors typical standard curve testing data aaa13144_td elisa kit vascular endothelial growth factor b vegf isoform vegfb 186 4507887 np_003368.1 nm_003377.4 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6 sensitivity up to 5 intra precision <= 8 inter 12 vegfb186 to5 <=8 inter12
⇄⧉products_description => string (827) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[6]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine VEGF monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄products_references => string (3) "N/A"
$value[6]['_source']['products_references']
⇄products_related_diseases => string (3) "N/A"
$value[6]['_source']['products_related_diseases']
⇄products_categories => string (3) "N/A"
$value[6]['_source']['products_categories']
⇄ncbi_full_name => string (3) "N/A"
$value[6]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value[6]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value[6]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value[6]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value[6]['_source']['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value[6]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value[6]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (3) "N/A"
$value[6]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[6]['_source']['ncbi_pathways']
⇄sp_protein_name => string (3) "N/A"
$value[6]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[6]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value[6]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value[6]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (3) "N/A"
$value[6]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[6]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[6]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
$value[6]['_source']['products_url']
⇄products_viewed => string (1) "0"
$value[6]['_source']['products_viewed']
⇄⧉search_terms => string (332) "aaa22610 canine no cross reaction with other factors typical standard curve ...
$value[6]['_source']['search_terms']
aaa22610 canine no cross reaction with other factors typical standard curve testing data aaa22610_td elisa kit vascular endothelial cell growth factor vegf samples serum plasma or culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6pg sensitivity up to 5 intra precision <= 8 inter 12 to5 <=8 inter12
⇄specificity => string (75) "Specifically recognize VEGF, no obvious cross reaction with other analogues"
$value[7]['_source']['specificity']
⇄purity => string (3) "N/A"
$value[7]['_source']['purity']
⇄form => string (3) "N/A"
$value[7]['_source']['form']
⇄concentration => string (3) "N/A"
$value[7]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[7]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Sandwich!!Samples||Serum, plasma, cell culture supernatant and other biological samples!!Detection Range||1.563-100pg/ml!!Sensitivity||0.938pg/ml
⇄⧉etc_term2 => string (270) "Intra-assay Precision||Intra-assay Precision: samples with low, medium and h...
$value[7]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.!!Inter-assay Precision||Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
⇄⧉products_description => string (1740) "Background: Vascular Endothelial Growth Factor (VEGF) is a growth factor tha...
$value[7]['_source']['products_description']
Background: Vascular Endothelial Growth Factor (VEGF) is a growth factor that plays an important role in angiogenesis and regulation of vascular permeability. It promotes the proliferation and migration of endothelial cells and also stimulates angiogenesis and maintains mature vascular networks. The function of VEGF is involved in many physiological and pathological processes, including embryonic development, tumor growth and metastasis, inflammation, heart disease, and neurological diseases. VEGF has several different subtypes, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, and VEGF-E. Among them, VEGF-A is the most extensively studied subtype and has various spliced variants with different biological functions. VEGF-A acts by binding to VEGF receptors, such as VEGFR1 and VEGFR2.<br><br>Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti VEGF antibody was precoated onto the 96-well plate. The biotin conjugated anti VEGF antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with VEGF conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of VEGF in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
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aaa17717 rabbit this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17717_sc elisa kit vascular endothelial cell growth factor samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 15.625 1000pg ml 9.375pg intra precision cv<8 inter cv<10
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This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
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Assay Type||Quantitative Competitive or Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
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$value[8]['_source']['products_description']
Principle of the Assay: VEGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VEGF. Standards or samples are then added to the microtiter plate wells and VEGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VEGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VEGF are added to each well to "sandwich" the VEGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (707) "aaa16218 rat this assay has high sensitivity and excellent specificity for d...
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aaa16218 rat this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16218_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type competitive sandwich 1.0 pg ml sandwich1.0
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This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: VEGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VEGF antibody and an VEGF-HRP conjugate. The assay sample and buffer are incubated together with VEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF concentration since VEGF from samples and VEGF-HRP conjugate compete for the anti-VEGF antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF from the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa17008 bovine this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17008_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
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This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: VEGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VEGF antibody and an VEGF-HRP conjugate. The assay sample and buffer are incubated together with VEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF concentration since VEGF from samples and VEGF-HRP conjugate compete for the anti-VEGF antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF from the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa17023 porcine this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17023_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
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This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: VEGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VEGF. Standards or samples are then added to the microtiter plate wells and VEGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VEGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VEGF are added to each well to "sandwich" the VEGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (698) "aaa16833 canine this assay has high sensitivity and excellent specificity fo...
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aaa16833 canine this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16833_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative sandwich 1.0pg ml
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This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
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Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep VEGF. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: VEGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VEGF antibody and an VEGF-HRP conjugate. The assay sample and buffer are incubated together with VEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF concentration since VEGF from samples and VEGF-HRP conjugate compete for the anti-VEGF antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF from the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.
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aaa16847 sheep this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16847_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
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This assay has high sensitivity and excellent specificity for detection of rabbit VEGF. No significant cross-reactivity or interference between rabbit VEGF and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa14964 rabbit this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa14964_td elisa kit vascular endothelial cell growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 samples serum plasma tissue homogenates type quantitative sandwich range 1.56 pg ml 100 < 0.39 intra precision within an cv ml100
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of monkey VEGF. No significant cross-reactivity or interference between monkey VEGF and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (596) "aaa15831 monkey this assay has high sensitivity and excellent specificity fo...
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aaa15831 monkey this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15831_td elisa kit vascular endothelial cell growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 samples serum plasma culture supernates lysates type quantitative sandwich range 125 pg ml 8000 < 31.25 intra precision within an cv range125
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of V...
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This assay has high sensitivity and excellent specificity for detection of VEGF-A. No significant cross-reactivity or interference between VEGF-A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF-A and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1416) "Principle of the Assay: VEGF-A ELISA kit applies the competitive enzyme immu...
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Principle of the Assay: VEGF-A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VEGF-A antibody and an VEGF-A-HRP conjugate. The assay sample and buffer are incubated together with VEGF-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF-A concentration since VEGF-A from samples and VEGF-A-HRP conjugate compete for the anti-VEGF-A antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF-A from the sample, fewer sites are left to bind VEGF-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF-A concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF-A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey VEGF-A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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Bladder Cancer Pathway||1030899!!Bladder Cancer Pathway||527!!Cytokine-cytokine Receptor Interaction Pathway||1030752!!Cytokine-cytokine Receptor Interaction Pathway||460!!Focal Adhesion Pathway||1030785!!Focal Adhesion Pathway||478!!HIF-1 Signaling Pathway||1030754!!MicroRNAs In Cancer Pathway||1030890!!MicroRNAs In Cancer Pathway||852928!!PI3K-Akt Signaling Pathway||1030766
⇄⧉search_terms => string (654) "aaa17213 monkey this assay has high sensitivity and excellent specificity fo...
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aaa17213 monkey this assay has high sensitivity and excellent specificity for detection of vegf a no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17213_sc elisa kit vascular endothelial growth factor vegfa vpf angiogenic permeability 17,247 da vegfa_sheep 68341933 np_001020281.1 p50412 nm_001025110.1 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉etc_term1 => string (174) "Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue hom...
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Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Competitive or Sandwich!!Detection Range||50-1000pg/mL!!Sensitivity||1.0pg/mL
⇄⧉etc_term2 => string (199) "Intended Uses||This VECGF ELISA kit is a 1.5 hour solid-phase ELISA designed...
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Intended Uses||This VECGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VECGF. This ELISA kit for research use only, not for therapeutic applications!
⇄⧉products_description => string (1188) "<b>Principle of the assay: </b>VECGF ELISA kit applies the competitive enzym...
$value[16]['_source']['products_description']
<b>Principle of the assay: </b>VECGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VECGF antibody and an VECGF-HRP conjugate. The assay sample and buffer are incubated together with VECGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VECGF concentration since VECGF from samples and VECGF-HRP conjugate compete for the anti-VECGF antibody binding site. Since the number of sites is limited, as more sites are occupied by VECGF from the sample, fewer sites are left to bind VECGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VECGF concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (453) "aaa16761 human typical testing data standard curve for reference only aaa167...
$value[16]['_source']['search_terms']
aaa16761 human typical testing data standard curve for reference only aaa16761_td elisa kit vascular endothelial cell growth factor vegf165 cancer samples serum plasma culture supernatants body fluid and tissue homogenate assay type competitive or sandwich detection range 50 1000pg ml sensitivity 1.0pg intended uses this vecgf is a 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications! range50 a1.5
⇄⧉products_description => string (1104) "Intended Uses: This immunoassay kit allows for the in vitro quantitative det...
$value[17]['_source']['products_description']
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (422) "aaa23328 human recombinant and natural vegf b typical testing data standard ...
$value[17]['_source']['search_terms']
aaa23328 human recombinant and natural vegf b typical testing data standard curve for reference only aaa23328_sc elisa kit vascular endothelial growth factor related vrf vegfb isoform 167 vegfl 21,261 da vegfb_human 344179103 np_001230662.1 p49765 nm_001243733.1 q16528 601398 samples serum plasma tissue homogenates cell culture supernates or other biological fluids detection range 15.6 1000 pg ml sensitivity isoform167
⇄⧉specificity => string (177) "This assay has high sensitivity and excellent specificity for detection of r...
$value[18]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of rat VEGF. No significant cross-reactivity or interference between rat VEGF and analogues was observed.
⇄purity => string (3) "N/A"
$value[18]['_source']['purity']
⇄form => string (3) "N/A"
$value[18]['_source']['form']
⇄concentration => string (3) "N/A"
$value[18]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[18]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (326) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[18]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[18]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄products_references => string (3) "N/A"
$value[18]['_source']['products_references']
⇄⧉products_related_diseases => string (193) "Heart Diseases||11!!Nervous System Diseases||8!!Hypertrophy||7!!Edema||6!!In...
⇄⧉search_terms => string (652) "aaa15591 mouse this assay has high sensitivity and excellent specificity for...
$value[18]['_source']['search_terms']
aaa15591 mouse this assay has high sensitivity and excellent specificity for detection of rat vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15591_td elisa kit vascular endothelial growth factor b cell vegfl vrf vegfb isoform b167 related 21,914 da vegfb_mouse 297632418 np_001172093.1 p49766 nm_001185164.1 q3ug04 q5d0b1 q64290 samples serum plasma tissue homogenates type quantitative sandwich range 3.9 pg ml 250 0.97 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in range3.9 ml250
⇄⧉products_description => string (676) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[19]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat VEGF-A monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄⧉search_terms => string (488) "aaa22456 goat no cross reaction with other factors typical testing data stan...
$value[19]['_source']['search_terms']
aaa22456 goat no cross reaction with other factors typical testing data standard curve for reference only aaa22456_sc elisa kit vascular endothelial growth factor a vegf vegfa vpf mvcd1 permeability 34,406 da vegfa_human 17380528 p15692.2 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6 sensitivity up to 5 intra precision to5
