Rabbit anti-Human RBM5 Polyclonal Antibody | anti-RBM5 antibody

RBM5, NT (RBM5, RNA-binding protein 5, Protein G15, Putative tumor suppressor LUCA15, RNA-binding motif protein 5, Renal carcinoma antigen NY-REN-9) (PE)

Cat. Num. AAA6340838

• Gene Names: RBM5; G15; H37; RMB5; LUCA15
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBM5; Polyclonal Antibody; NT (RBM5; RNA-binding protein 5; Protein G15; Putative tumor suppressor LUCA15; RNA-binding motif protein 5; Renal carcinoma antigen NY-REN-9) (PE); Renal carcinoma antigen NY-REN-9; anti-RBM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RBM5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RBM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RBM5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RBM5 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RBM5 antibody detected the RBM5 protein (arrow).)

Related Product Information for anti-RBM5 antibody

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

Product Categories/Family for anti-RBM5 antibody

Antibodies; Cancer Markers

References

Sugliani, M., et al. Plant Cell 22(6):1936-1946(2010). Rintala-Maki, N.D., et al. Gene 445 (1-2), 7-16 (2009): Kotlajich, M.V., et al. Mol. Cell 32(2):162-164(2008). Fushimi, K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(41):15708-15713(2008). Bonnal, S., et al. Mol. Cell 32(1):81-95(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5032031
• NCBI GeneID: 10181
• NCBI Accession #: NP_005769.1
• NCBI GenBank Nucleotide #: NM_005778.3
• UniProt Accession #: P52756; Q93021; Q9BU14; Q9HDA6; Q9UKY8; Q9UL24; B2RA45; B4DM16; B4DMF9; B4DZ63
• Molecular Weight: 92,154 Da
• NCBI Official Full Name: RNA-binding protein 5
• NCBI Official Synonym Full Names: RNA binding motif protein 5
• NCBI Official Symbol: RBM5
• NCBI Official Synonym Symbols: G15; H37; RMB5; LUCA15
• NCBI Protein Information: RNA-binding protein 5; putative tumor suppressor LUCA15; renal carcinoma antigen NY-REN-9
• UniProt Protein Name: RNA-binding protein 5
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM5
• UniProt Entry Name: RBM5_HUMAN

NCBI Description

This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]

Uniprot Description

RBM5: Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. Belongs to the RBM5/RBM10 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; RNA splicing; C2H2-type zinc finger protein; RNA processing; Apoptosis; RNA-binding

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: nucleoplasm; spliceosome; nucleus

Molecular Function: mRNA binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; negative regulation of cell proliferation; apoptosis; positive regulation of apoptosis; RNA splicing; spliceosome assembly; gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome