FCM/FACS (Flow Cytometry)
(Separation of human CD173 positive blood debris (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD173 Antibody | anti-CD173 antibody
Anti-Hu CD173 PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD173; N/A; Anti-Hu CD173 PE; Lewis Y; blood group H2 antigen; LeY; anti-CD173 antibody
Reactivity
Human
Isotype
Mouse IgM
Clone Number
MEM-195
Specificity
The antibody MEM-195 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.
Form/Format
PE
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Applicable Applications for anti-CD173 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Human thrombocytes
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD173 positive blood debris (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD173 positive blood debris (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD173 antibody
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
References
*Moehler TM, Sauer S, Witzel M, Andrulis M, Garcia-Vallejo JJ, Grobholz R, Willhauck-Fleckenstein M, Greiner A, Goldschmidt H, Schwartz-Albiez R: Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface-expressed Lewis(y)(CD174) in first endothelial cell-cell contacts during angiogenesis. J Cell Physiol. 2008 Apr;215(1):27-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18205178?itool,*Cao Y, Merling A, Karsten U, Schwartz-Albiez R: The fucosylated histo-blood group antigens H type 2 (blood group O, CD173) and Lewis Y (CD174) are expressed on CD34+ hematopoietic progenitors but absent on mature lymphocytes. Glycobiology. 2001 Aug;11(8):677-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/11479278?itool,
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Product Notes
The CD173 (Catalog #AAA129290) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD173 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD173 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD173 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD173, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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