Rabbit anti-Human RBL1 Polyclonal Antibody | anti-RBL1 antibody

RBL1, CT (RBL1, Retinoblastoma-like protein 1, 107kD retinoblastoma-associated protein, pRb1) (APC)

Cat. Num. AAA6340642

• Gene Names: RBL1; PRB1; p107; CP107
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBL1; Polyclonal Antibody; CT (RBL1; Retinoblastoma-like protein 1; 107kD retinoblastoma-associated protein; pRb1) (APC); pRb1; anti-RBL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RBL1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from the C-terminal region of human RBL1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RBL1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RBL1 antibody detected the RBL1 protein (arrow).)

Related Product Information for anti-RBL1 antibody

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-RBL1 antibody

Antibodies; Retinoblastoma Proteins

References

Jayadeva, G., et al. J. Biol. Chem. 285(39):29863-29873(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 34577079
• NCBI GeneID: 5933
• NCBI Accession #: NP_002886.2
• NCBI GenBank Nucleotide #: NM_002895.2
• UniProt Accession #: P28749; Q4VXA0; Q8N5K6; Q9H1L5; Q9H1M1; A8K2W5
• Molecular Weight: 120,847 Da
• NCBI Official Full Name: retinoblastoma-like protein 1 isoform a
• NCBI Official Synonym Full Names: retinoblastoma-like 1 (p107)
• NCBI Official Symbol: RBL1
• NCBI Official Synonym Symbols: PRB1; p107; CP107
• NCBI Protein Information: retinoblastoma-like protein 1; cellular protein 107; 107 kDa retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-like protein 1
• Protein Family: RHOMBOID-like protein
• UniProt Gene Name: RBL1
• UniProt Synonym Gene Names: p107
• UniProt Entry Name: RBL1_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Rb-like 1: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor. Belongs to the retinoblastoma protein (RB) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: protein binding; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; regulation of lipid kinase activity; chromatin modification; negative regulation of transcription from RNA polymerase II promoter