Rabbit anti-Mouse RBC Polyclonal Antibody | anti-RBC antibody
Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)
Reactivity
Mouse
Applications
Cytotoxicity Assay
Synonyms
RBC; Polyclonal Antibody; Anti-Mouse RBC; IgM Fraction; (Polyclonal) (rabbit IgM); anti-RBC antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgM
Specificity
RBCs
Form/Format
5 mg of IgM fraction in 0.5M NaCl, 0.01M phosphate.
Concentration
0.75 mg IgM/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-RBC antibody
Cytotoxicity Assay (CT)
Application Notes
Optimum conditions for use of this antibody in analytical procedures should be established in each laboratory.
Preservatives
0.2 um sterile filtered; contains 0.05% sodium azide.
Hemagglutination
- Test Cell Suspension: 100 ul of washed 1.5% fresh mouse RBCs in saline.
- Product Tested: Diluted antibody (lot#: 0192M-1) in 100 ul of heat inactivated normal rabbit serum diluted 1:100 in saline.
- Incubation: 37°C for 20 mins, in U-bottom microtiter plates, 20°C for 2 to 4 hrs. for cell settling pattern
- Titer: The highest dilution of the product that yields agglutination.
- Results: Titer of 1:400
- Product Tested: Diluted antibody (lot#: 0192M-1) in 100 ul of heat inactivated normal rabbit serum diluted 1:100 in saline.
- Incubation: 37°C for 20 mins, in U-bottom microtiter plates, 20°C for 2 to 4 hrs. for cell settling pattern
- Titer: The highest dilution of the product that yields agglutination.
- Results: Titer of 1:400
Ouchterlony
- Sample Size: 25 ul
- Distance between wells: 4 mm
- Test time: 24 hr.
- Antiserum tested: Goat anti-rabbit IgM and Goat anti-rabbit IgG
- Results: Positive for IgM and Negative for IgG
- Distance between wells: 4 mm
- Test time: 24 hr.
- Antiserum tested: Goat anti-rabbit IgM and Goat anti-rabbit IgG
- Results: Positive for IgM and Negative for IgG
Preparation and Storage
Divide into useful aliquots and store at 2-8°C.
Related Product Information for anti-RBC antibody
This product is rabbit IgM directed against mouse red blood cells. This IgM fraction was isolated fiom a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for immunoglobulin purity and protein concentration. This IgM fraction contains agglutinating and lysing antibodies and can be used for these procedures.
Product Categories/Family for anti-RBC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Ribulose bisphosphate carboxylase
NCBI Official Symbol
TK_RS11535
NCBI Protein Information
ribulose bisophosphate carboxylase
UniProt Protein Name
Ribulose bisphosphate carboxylase
UniProt Gene Name
rbcL
UniProt Synonym Gene Names
RuBisCO
UniProt Entry Name
RBL_THEKO
Uniprot Description
Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA) (PubMed:9988755, PubMed:10512715). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase (PubMed:17303759).
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Product Notes
The RBC rbcl (Catalog #AAA524365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RBC can be used in a range of immunoassay formats including, but not limited to, Cytotoxicity Assay (CT). Optimum conditions for use of this antibody in analytical procedures should be established in each laboratory. Researchers should empirically determine the suitability of the RBC rbcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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