Immunohistochemistry (IHC)
(MBS9603690 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit RBBP9 Polyclonal Antibody | anti-RBBP9 antibody
RBBP9 Antibody
Gene Names
RBBP9; BOG; RBBP10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RBBP9; Polyclonal Antibody; RBBP9 Antibody; B5T overexpressed gene protein; B5T-overexpressed gene protein; BOG; Bog protein; MGC9236; OTTHUMP00000030360; Protein BOG; Putative hydrolase RBBP9; RBBP 10; RBBP 9; RBBP-10; RBBP-9; RBBP10; RBBP9_HUMAN; Retinoblastoma binding protein 10; Retinoblastoma binding protein 9; Retinoblastoma-binding protein 10; Retinoblastoma-binding protein 9; Retinoma binding protein 9; anti-RBBP9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RBBP9 antibody detects endogenous levels of RBBP9.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
186
Applicable Applications for anti-RBBP9 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human RBBP9.
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (82%), Bovine (83%), Horse (100%), Sheep (92%), Rabbit (92%), Dog (92%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603690 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603690 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of A375 cells, using RBBP9 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of A375 cells, using RBBP9 antibody.)
Related Product Information for anti-RBBP9 antibody
Function: May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
Similarity: Belongs to the RBBP9 family.
Similarity: Belongs to the RBBP9 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 21 kDa
Predicted: 21 kDa
NCBI Official Full Name
putative hydrolase RBBP9
NCBI Official Synonym Full Names
RB binding protein 9, serine hydrolase
NCBI Official Symbol
RBBP9
NCBI Official Synonym Symbols
BOG; RBBP10
NCBI Protein Information
putative hydrolase RBBP9
UniProt Protein Name
Putative hydrolase RBBP9
Protein Family
UniProt Gene Name
RBBP9
UniProt Synonym Gene Names
BOG; RBBP10; Protein BOG; RBBP-10; RBBP-9
NCBI Description
The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
Research Articles on RBBP9
Similar Products
Product Notes
The RBBP9 rbbp9 (Catalog #AAA9603690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBBP9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RBBP9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the RBBP9 rbbp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBBP9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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