Western Blot (WB)
Rabbit anti-Human, Mouse RB1 Polyclonal Antibody | anti-RB1 antibody
Anti-RB1 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
RB1; Polyclonal Antibody; Anti-RB1 Antibody; anti-RB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Western blot analysis of Jurkat cell and A431 cell using RB1 antibody.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IHC: 1:50 - 1:100
Immunogen
Recombinant protein of human RB1
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles. degree
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-RB1 antibody
The retinoblastoma tumor suppressor protein, RB1, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). RB1 has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits RB1 target binding and allows cell cycle progression (5). RB1 inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
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Product Notes
The RB1 (Catalog #AAA419365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RB1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:1000 IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the RB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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