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Dot Blot (DB) (Dot blot analysis of anti-hRb-S811 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)

Rabbit anti-Human RB1 Polyclonal Antibody | anti-RB1 antibody

RB1, phosphorylated (Ser811) (Retinoblastoma-associated Protein, pRb, Rb, PP110, P105-Rb)

Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Gel Super Shift Assay
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
RB1; Polyclonal Antibody; phosphorylated (Ser811) (Retinoblastoma-associated Protein; pRb; Rb; PP110; P105-Rb); Anti -RB1; anti-RB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RB when phosphorylated at Ser811.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-RB1 antibody
ELISA (EL/EIA), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, Dot Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Dot Blot: 1:500
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser811 of human RB (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-hRb-S811 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)

Dot Blot (DB) (Dot blot analysis of anti-hRb-S811 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-RB1 antibody
Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression occurs in some cases.
Product Categories/Family for anti-RB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
106,159 Da
NCBI Official Full Name
retinoblastoma-associated protein
NCBI Official Synonym Full Names
retinoblastoma 1
NCBI Official Symbol
RB1
NCBI Official Synonym Symbols
RB; pRb; OSRC; pp110; p105-Rb
NCBI Protein Information
retinoblastoma-associated protein; retinoblastoma suspectibility protein
UniProt Protein Name
Retinoblastoma-associated protein
UniProt Gene Name
RB1
UniProt Synonym Gene Names
Rb
UniProt Entry Name
RB_HUMAN

NCBI Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Transcription, coactivator/corepressor; Tumor suppressor; Oncoprotein; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; nucleus; chromatin

Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor activity; transcription factor binding

Biological Process: sister chromatid biorientation; viral reproduction; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; enucleate erythrocyte differentiation; regulation of lipid kinase activity; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; Ras protein signal transduction; androgen receptor signaling pathway; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle

Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma

Research Articles on RB1

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Product Notes

The RB1 rb1 (Catalog #AAA624562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RB1, phosphorylated (Ser811) (Retinoblastoma-associated Protein, pRb, Rb, PP110, P105-Rb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Dot Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Immunohistochemistry: 1:50-1:100 Dot Blot: 1:500. Researchers should empirically determine the suitability of the RB1 rb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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