Rabbit anti-Human RASGRF1 Polyclonal Antibody | anti-RASGRF1 antibody

RASGRF1 (S929) (Ras-specific Guanine Nucleotide-releasing Factor 1, Ras-GRF1, Guanine Nucleotide-releasing Protein, Ras-specific Nucleotide Exchange Factor CDC25, CDC25, GNRP, GRF1) (FITC)

Cat. Num. AAA6479301

• Gene Names: RASGRF1; GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RASGRF1; Polyclonal Antibody; RASGRF1 (S929) (Ras-specific Guanine Nucleotide-releasing Factor 1; Ras-GRF1; Guanine Nucleotide-releasing Protein; Ras-specific Nucleotide Exchange Factor CDC25; CDC25; GNRP; GRF1) (FITC); anti-RASGRF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RASGRF1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RASGRF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S929 of human RASGRF1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RASGRF1-pS929 in MDA-MB231 cell line lysates (35ug/lane). RASGRF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 Antibody (S929), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-RASGRF1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Iguchi, T., Int. J. Oncol. 31(2), 285-291 (2007). 2. Holmes, R., Mol. Cell. Biol. 26(24), 9564-9570 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 24797099
• NCBI GeneID: 5923
• NCBI Accession #: NP_002882
• NCBI GenBank Nucleotide #: NM_002891
• UniProt Accession #: Q13972
• Molecular Weight: 145kDa
• NCBI Official Full Name: ras-specific guanine nucleotide-releasing factor 1 isoform 1
• NCBI Official Synonym Full Names: Ras protein specific guanine nucleotide releasing factor 1
• NCBI Official Symbol: RASGRF1
• NCBI Official Synonym Symbols: GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
• NCBI Protein Information: ras-specific guanine nucleotide-releasing factor 1
• UniProt Protein Name: Ras-specific guanine nucleotide-releasing factor 1
• Protein Family: Ras-specific guanine nucleotide-releasing factor
• UniProt Gene Name: RASGRF1
• UniProt Synonym Gene Names: CDC25; GNRP; GRF1; Ras-GRF1; GNRP
• UniProt Entry Name: RGRF1_HUMAN

NCBI Description

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Uniprot Description

RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported.

Protein type: GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: neuron projection; growth cone; plasma membrane; cytosol

Molecular Function: glutamate receptor binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: regulation of Ras protein signal transduction; synaptic transmission; regulation of Rac protein signal transduction; cell proliferation; long-term memory; small GTPase mediated signal transduction; regulation of synaptic plasticity; positive regulation of Ras protein signal transduction; signal transduction; neurite development; regulation of neuronal synaptic plasticity