Rabbit RasGAP Polyclonal Antibody | anti-RasGAP antibody

RasGAP (Ras GTPase Activating Protein, GAP) (PE)

Cat. Num. AAA6497029

• Gene Names: RASA1; GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• Reactivity: Human, Chicken, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RasGAP; Polyclonal Antibody; RasGAP (Ras GTPase Activating Protein; GAP) (PE); anti-RasGAP antibody

• Host: Rabbit
• Reactivity: Human, Chicken, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAP at 120kD. Species Crossreactivity: chicken, mouse and rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RasGAP antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 5ug. Immunoprecipitates GAP protein in a RIPA lysate of human Raji lymphocyte cells.; WB: 0.5-2ug/m. Detects GAP protein in a RIPA lysate of human Raji lymphocyte cells, rat L6 myoblasts lysate and mouse Wehi leukemia cell lysate.; Applications are based on unconjugated antibody.
• Immunogen: Trp E-fusion protein containing aa171-448 of human GAP.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-RasGAP antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Ellis, et al., Nature 343: 377 (1990). 2. Yoder-Hill, et al., JBC 270: 27615 (1995). 3. Abdellatif, M., Schneider, M.D.: An effector-like function of Ras GTPase-activating protein predominates in cardiac muscle cells. J. Biol. Chem. 272: 525-33 (1997). 4. Levy-Toledano, R., et al. : Insulin-induced activation of phosphatidylinositol (PI) 3-kinase. Insulin-induced phosphorylation of insulin receptors and insulin receptor substrate-1 displaces phosphorylated platelet-derived growth factor receptors from binding sites on PI 3-kinase. J. Biol. Chem. 270: 30018-22 (1995). 5. Liu, M., et al. : Mechanical strain induces pp60src activation and translocation to cytoskeleton in fetal rat lung cells. J. Biol. Chem. 271: 7066-71 (1996). 6. Owen-Lynch, P.J., et al. : v-Abl-mediated apoptotic suppression is associated with SHC phosphorylation without concomitant mitogen-activated protein kinase activation. J. Biol. Chem. 270: 5956-62 (1995). 7. Ruff, S.J., et al. : Peroxovanadate induces tyrosine phosphorylation of multiple signaling proteins in mouse liver and kidney. J. Biol. Chem. 272: 1263-7 (1997). 8. Taylor, S.J., et al. : Functional interaction between c-Src and its mitotic target, Sam 68. J. Biol. Chem 270: 10120-4 (1995). 9. Yoder-Hill, J., et al. : A conserved region of c-Ha-Ras is required for efficient GTPase stimulation by GTPase activating protein but not neurofibromin. J. Biol. Chem 270: 27615-21 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 121743
• NCBI GeneID: 5921
• NCBI Accession #: P20936.1
• NCBI GenBank Nucleotide #: NM_022650
• UniProt Accession #: P20936; Q9UDI1; B2R6W3
• Molecular Weight: 1047
• NCBI Official Full Name: Ras GTPase-activating protein 1
• NCBI Official Synonym Full Names: RAS p21 protein activator (GTPase activating protein) 1
• NCBI Official Symbol: RASA1
• NCBI Official Synonym Symbols: GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• NCBI Protein Information: ras GTPase-activating protein 1; triphosphatase-activating protein
• UniProt Protein Name: Ras GTPase-activating protein 1
• Protein Family: RasGAP-activating-like protein
• UniProt Gene Name: RASA1
• UniProt Synonym Gene Names: GAP; RASA; GAP; GTPase-activating protein; RasGAP
• UniProt Entry Name: RASA1_HUMAN

NCBI Description

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]

Uniprot Description

RASA1: a GTPase activator for normal RAS p21 but not its oncogenic counterpart, converting it to the putatively inactive GDP-bound state. Acting as a suppressor of RAS function, it enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; GAPs, Ras; Oncoprotein; GAPs

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: ruffle; cytoplasm; cytosol

Molecular Function: GTPase binding; protein binding; glycoprotein binding; potassium channel inhibitor activity; receptor binding

Biological Process: axon guidance; negative regulation of cell adhesion; negative regulation of cell-matrix adhesion; signal transduction; regulation of cell shape; regulation of actin filament polymerization; embryonic development; cytokinesis after mitosis; regulation of RNA metabolic process; ephrin receptor signaling pathway; blood vessel morphogenesis; negative regulation of Ras protein signal transduction; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; vasculogenesis

Disease: Parkes Weber Syndrome; Capillary Malformation-arteriovenous Malformation; Basal Cell Carcinoma, Susceptibility To, 1