Rabbit anti-Human RASD2 Polyclonal Antibody | anti-RASD2 antibody

RASD2, ID (RASD2, TEM2, GTP-binding protein Rhes, Ras homolog enriched in striatum, Tumor endothelial marker 2) (Azide free) (HRP)

Cat. Num. AAA6340442

• Gene Names: RASD2; Rhes; TEM2; MGC:4834
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASD2; Polyclonal Antibody; ID (RASD2; TEM2; GTP-binding protein Rhes; Ras homolog enriched in striatum; Tumor endothelial marker 2) (Azide free) (HRP); Tumor endothelial marker 2; anti-RASD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RASD2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RASD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the Central region of human RASD2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RASD2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RASD2 antibody detected the RASD2 protein (arrow).)

Related Product Information for anti-RASD2 antibody

RASD2 is a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades.

Product Categories/Family for anti-RASD2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Hill, C., et al. Cell. Physiol. Biochem. 23 (1-3), 1-8 (2009): Liu, Y.L., et al. Biol. Psychiatry 64(9):789-796(2008). Thapliyal, A., et al. Am. J. Physiol., Cell Physiol. 295 (5), C1417-C1426 (2008): Agretti, P., et al. J. Endocrinol. Invest. 30(4):279-284(2007). Taylor, J.P., et al. Biochem. Biophys. Res. Commun. 349(2):809-815(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 22027486
• NCBI GeneID: 23551
• NCBI Accession #: NP_055125.2
• NCBI GenBank Nucleotide #: NM_014310.3
• UniProt Accession #: Q96D21; O95520; Q5THY8
• Molecular Weight: 30,366 Da
• NCBI Official Full Name: GTP-binding protein Rhes
• NCBI Official Synonym Full Names: RASD family, member 2
• NCBI Official Symbol: RASD2
• NCBI Official Synonym Symbols: Rhes; TEM2; MGC:4834
• NCBI Protein Information: GTP-binding protein Rhes; tumor endothelial marker 2; Ras homolog enriched in striatum
• UniProt Protein Name: GTP-binding protein Rhes
• Protein Family: GTP-binding protein
• UniProt Gene Name: RASD2
• UniProt Synonym Gene Names: TEM2
• UniProt Entry Name: RHES_HUMAN

NCBI Description

This gene encodes a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades. [provided by RefSeq, Jul 2008]

Uniprot Description

RASD2: GTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein, monomeric, RasD; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: plasma membrane

Molecular Function: GTPase activity; GTP binding; ubiquitin conjugating enzyme binding; G-protein beta-subunit binding

Biological Process: positive regulation of protein sumoylation; positive regulation of protein kinase B signaling cascade; synaptic transmission, dopaminergic; metabolic process; small GTPase mediated signal transduction; locomotory behavior; negative regulation of protein ubiquitination