Western Blot (WB)
(Western Blot (WB) analysis of RARS polyclonal antibody)
Rabbit RARS Polyclonal Antibody | anti-RARS antibody
RARS Polyclonal Antibody
Gene Names
RARS1; HLD9; RARS; ArgRS; DALRD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RARS; Polyclonal Antibody; RARS Polyclonal Antibody; Arginine--tRNA ligase; cytoplasmic; Arginyl-tRNA synthetase; ArgRS; anti-RARS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RARS polyclonal antibody detects endogenous levels of RARS protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
660
Applicable Applications for anti-RARS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
IHC: 1:50-1:200
IF: 1:10-1:100
Immunogen
Recombinant full length Human RARS.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of RARS polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of RARS polyclonal antibody)
Related Product Information for anti-RARS antibody
The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. ArgRS (Arginyl-tRNA synthetase), also known as RARS or DALRD1, belongs to the class-I aminoacyl-tRNA synthetase family that includes the related proteins, LeuRS, ValRS and IleRS. These proteins are large monomeric proteins and play a major role in catalyzing the aminoacylation of tRNA by their cognate amino acid. ArgRS localizes to the cytoplasm and exists as a monomer but can also associate with other tRNA synthetases and auxiliary proteins to form a multisubunit complex. In the presence of ATP, arginine (Arg) and tRNA, ArgRS joins Arg to tRNA(Arg) at its synthetic active site. Two cytoplasmic forms of ArgRS have been described in mammals, differing by the addition of a 73 amino acid sequence that is required for ArgRS assembly into the multisubunit complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 75kDa
NCBI Official Full Name
arginine--tRNA ligase, cytoplasmic
NCBI Official Synonym Full Names
arginyl-tRNA synthetase 1
NCBI Official Symbol
RARS1
NCBI Official Synonym Symbols
HLD9; RARS; ArgRS; DALRD1
NCBI Protein Information
arginine--tRNA ligase, cytoplasmic
UniProt Protein Name
Arginine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
RARS
UniProt Synonym Gene Names
ArgRS
UniProt Entry Name
SYRC_HUMAN
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]
Research Articles on RARS
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Product Notes
The RARS rars (Catalog #AAA3011497) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RARS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the RARS rars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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