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Western Blot (WB)
(RARS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).)
Rabbit anti-Human RARS Polyclonal Antibody | anti-RARS antibody
RARS, CT (RARS, Arginine-tRNA ligase, cytoplasmic, Arginyl-tRNA synthetase) (MaxLight 405)
Gene Names
RARS; HLD9; ArgRS; DALRD1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
RARS; Polyclonal Antibody; CT (RARS; Arginine-tRNA ligase; cytoplasmic; Arginyl-tRNA synthetase) (MaxLight 405); Arginine--tRNA ligase; Arginyl-tRNA synthetase; anti-RARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-RARS antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 612-642 amino acids from the C-terminal region of human RARS.
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RARS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).)
Western Blot (WB)
(RARS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).)
Immunohistochemistry (IHC)
(RARS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RARS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of RARS Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of RARS Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(RARS Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RARS Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RARS antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq].
Product Categories/Family for anti-RARS antibody
References
Bottoni, A., et al. J. Cell. Physiol. 212(2):293-297(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005). Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003). Kaminska, M., et al. Biochemistry 40(47):14309-14316(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,140 Da
NCBI Official Full Name
arginine--tRNA ligase, cytoplasmic
NCBI Official Synonym Full Names
arginyl-tRNA synthetase
NCBI Official Symbol
RARS
NCBI Official Synonym Symbols
HLD9; ArgRS; DALRD1
NCBI Protein Information
arginine--tRNA ligase, cytoplasmic
UniProt Protein Name
Arginine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
RARS
UniProt Synonym Gene Names
ArgRS
UniProt Entry Name
SYRC_HUMAN
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]
Research Articles on RARS
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Product Notes
The RARS rars (Catalog #AAA6340366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RARS, CT (RARS, Arginine-tRNA ligase, cytoplasmic, Arginyl-tRNA synthetase) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RARS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IF: 1:10-50 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RARS rars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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