Rabbit RARbeta Polyclonal Antibody | anti-RARbeta antibody

RARbeta (L359) Polyclonal Antibody

Cat. Num. AAA3000846

• Gene Names: RARB; HAP; RRB2; NR1B2; MCOPS12; RARbeta1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RARbeta; Polyclonal Antibody; RARbeta (L359) Polyclonal Antibody; Retinoic acid receptor beta; RAR-beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon; RARB; HAP; NR1B2; anti-RARbeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Retinoic Acid Receptor beta (L359) polyclonal antibody detects endogenous levels of Retinoic Acid Receptor beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 448

• Applicable Applications for anti-RARbeta antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 321-370 of Human RARbeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RARbeta (L359) pAb at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RARbeta (L359) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-RARbeta antibody

Retinoids are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are nuclear transcription factors that modulate the effects of retinoids (RA) on gene expression. Most retinoid forms (including all trans RA, 9-cis RA, 4oxo RA and 3, 4 dihydro RA) activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RA binds RARs, inducing a change in receptor configuration that allows DNA binding and increased gene transcription from specific genes to occur. RAR family members, which include RARalpha, RARbeta and RARgamma, belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. Retinoid receptor expression is tissue specific; the skin expresses RARgamma and RXRalpha.

NCBI and Uniprot Product Information

• NCBI GI #: 14916494
• NCBI GeneID: 5915
• NCBI Accession #: NP_000956.2
• NCBI GenBank Nucleotide #: NP_000956.2
• UniProt Accession #: P10826
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: retinoic acid receptor beta isoform 1
• NCBI Official Synonym Full Names: retinoic acid receptor beta
• NCBI Official Symbol: RARB
• NCBI Official Synonym Symbols: HAP; RRB2; NR1B2; MCOPS12; RARbeta1
• NCBI Protein Information: retinoic acid receptor beta
• UniProt Protein Name: Retinoic acid receptor beta
• UniProt Gene Name: RARB
• UniProt Synonym Gene Names: HAP; NR1B2; RAR-beta
• UniProt Entry Name: RARB_HUMAN

NCBI Description

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. Alternate promoter usage and differential splicing result in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

RARB: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARB is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Four splice-variant isoforms have been described. Isoform beta-1 and beta-2 are nuclear and isoform beta-4 cytoplasmic.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor; Oncoprotein

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; protein complex binding; steroid hormone receptor activity; drug binding; retinoic acid receptor activity

Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of myelination; striatum development; glandular epithelial cell development; positive regulation of apoptosis; embryonic eye morphogenesis; multicellular organism growth; negative regulation of chondrocyte differentiation; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; embryonic hindlimb morphogenesis; negative regulation of cell proliferation; ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; positive regulation of neuron differentiation; transmembrane transport; embryonic gut development; negative regulation of apoptosis

Disease: Microphthalmia, Syndromic 12