Rabbit anti-Human, Mouse RAP2A Polyclonal Antibody | anti-RAP2A antibody

RAP2A Antibody

Cat. Num. AAA9630921

• Gene Names: RAP2A; KREV; RAP2; K-REV; RbBP-30
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RAP2A; Polyclonal Antibody; RAP2A Antibody; K REV; KREV; RAP2; member of RAS oncogene family; RAP2A_HUMAN; Ras-related protein Rap-2a; RbBP 30; RbBP-30; RbBP30; anti-RAP2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RAP2A Antibody detects endogenous levels of RAP2A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RAP2A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RAP2A.
• Conjugation: Unconjugated
• Similarity: The effector domain mediates the interaction with RUNDC3A. Belongs to the small GTPase superfamily. Ras family.
• Post Translational Modifications: Ubiquitinated; undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A.Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.
• Subunit Structure: Interacts (GTP-bound form) with RUNDC3A. Interacts with RGS14; the interaction is GTP-dependent (By similarity). Interacts with PLCE1. Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3. Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4. Interacts with MINK1. Interacts (GTP-bound form preferentially) with MAP4K4. Interacts with cytoskeletal actin.
• Function: Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.
• Note: May also localize to the Golgi (PubMed:7962206) and the gelatinase-containing granules of neutrophils (PubMed:8391995). Colocalizes with RASGEF1B to midbody at telophase (PubMed:23894443).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RAP2A antibody

Recycling endosome membrane; Lipid-anchor; Cytoplasmic side; Midbody; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 131852
• NCBI GeneID: 5911
• UniProt Accession #: P10114; Q5JSC1; Q5JSC2; B2RCJ1
• Molecular Weight: 183
• NCBI Official Full Name: Ras-related protein Rap-2a
• NCBI Official Synonym Full Names: RAP2A, member of RAS oncogene family
• NCBI Official Symbol: RAP2A
• NCBI Official Synonym Symbols: KREV; RAP2; K-REV; RbBP-30
• NCBI Protein Information: ras-related protein Rap-2a; RAP2, member of RAS oncogene family (K-rev)
• UniProt Protein Name: Ras-related protein Rap-2a
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP2A
• UniProt Entry Name: RAP2A_HUMAN

Uniprot Description

RAP2A: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: recycling endosome; recycling endosome membrane; plasma membrane; midbody; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: microvillus biogenesis; establishment of protein localization; metabolic process; regulation of dendrite morphogenesis; actin cytoskeleton reorganization; establishment of epithelial cell polarity; positive regulation of protein amino acid phosphorylation; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; regulation of JNK cascade; Rap protein signal transduction