Rabbit RANTES Polyclonal Antibody | anti-Ccl5 antibody

RABBIT ANTI MOUSE RANTES

Cat. Num. AAA221278

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: RANTES; Polyclonal Antibody; RABBIT ANTI MOUSE RANTES; anti-Ccl5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 91

• Applicable Applications for anti-Ccl5 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Maximum Dilution: 0.5ug/ml;; Functional Assays: Minimum Dilution: 6.0ug/ml; Maximum Dilution: 8.0ug/ml;; Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Immunogen: Recombinant mouse RANTES; Buffer Solution
• Antiserum Preparation: Antisera to mouse RANTES were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0. 09% sodium azide is recommended. N. B. For functional studies do not add azide.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-Ccl5 antibody

Rabbit anti Mouse RANTES antibody recognizes mouse RANTES, also known as CCL5. RANTES is expressed by circulating T cells and T cells in culture. RANTES is chemotactic for peripheral blood monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 164698428
• NCBI GeneID: 20304
• NCBI Accession #: NP_038681.2
• NCBI GenBank Nucleotide #: NM_013653.3
• UniProt Accession #: P30882
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; T-cell-specific protein RANTES; small inducible cytokine A5; small-inducible cytokine A5
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_MOUSE

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Cell adhesion; Chemokine; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: heparin binding; protein homodimerization activity; protein self-association; cytokine activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; CCR1 chemokine receptor binding; chemokine receptor binding; chemokine activity; chemokine receptor antagonist activity; chemoattractant activity; phospholipase activator activity; CCR5 chemokine receptor binding

Biological Process: positive regulation of cell adhesion; exocytosis; positive regulation of osteoclast differentiation; response to toxin; positive regulation of smooth muscle cell proliferation; chemotaxis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of homotypic cell-cell adhesion; pseudopodium formation; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of T cell proliferation; inflammatory response; lymphocyte chemotaxis; protein tetramerization; phospholipase D activation; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; positive regulation of cellular biosynthetic process; cellular calcium ion homeostasis; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cellular protein complex assembly; negative regulation of viral genome replication; positive regulation of tyrosine phosphorylation of STAT protein; response to cytokine stimulus; eosinophil chemotaxis; positive regulation of fever; immune response; positive regulation of neuron differentiation; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; regulation of insulin secretion; positive regulation of epithelial cell proliferation; positive regulation of phosphorylation; regulation of T cell activation; positive regulation of cell migration