Goat anti-Human, Mouse RanGAP 1 Polyclonal Antibody | anti-RANGAP1 antibody

RanGAP 1 (RANGAP1, KIAA1835, Ran GTPase activating protein 1, Fug1)

Cat. Num. AAA610023

• Gene Names: RANGAP1; SD; Fug1; RANGAP
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RanGAP 1; Polyclonal Antibody; RanGAP 1 (RANGAP1; KIAA1835; Ran GTPase activating protein 1; Fug1); Anti -RanGAP 1 (RANGAP1; anti-RANGAP1 antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical. Species Crossreactivity: human, mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-RANGAP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 70kD band observed in Human Brain and 3T3 lysates (predicted MW of 68kD according to NP_002874). Recommended for use at 0.1-0.5ug/ml., Staining (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.
• Immunogen: ASEDIAKLAETLAK-C, N Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-RANGAP1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4506411
• NCBI GeneID: 5905
• NCBI Accession #: NP_002874.1
• NCBI GenBank Nucleotide #: NM_002883.3
• UniProt Accession #: P46060; Q96JJ2
• Molecular Weight: 63,542 Da
• NCBI Official Full Name: ran GTPase-activating protein 1
• NCBI Official Synonym Full Names: Ran GTPase activating protein 1
• NCBI Official Symbol: RANGAP1
• NCBI Official Synonym Symbols: SD; Fug1; RANGAP
• NCBI Protein Information: ran GTPase-activating protein 1; segregation distortion; segregation distorter homolog
• UniProt Protein Name: Ran GTPase-activating protein 1
• Protein Family: RAN GTPase-activating protein
• UniProt Gene Name: RANGAP1
• UniProt Synonym Gene Names: KIAA1835; SD; RanGAP1
• UniProt Entry Name: RAGP1_HUMAN

NCBI Description

This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

RANGAP1: a cytoplasmic GTPase activator for the Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. Homodimerizes. Forms a tight complex in association with RANBP2 and the ubiquitin-conjugating enzyme E2 (UBC9).

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: kinetochore; spindle pole; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; nuclear pore; cytosol

Molecular Function: protein binding

Biological Process: negative regulation of protein export from nucleus; mitotic cell cycle; signal transduction