anti-Human RAMP1 Polyclonal Antibody | anti-RAMP1 antibody
Anti - RAMP1 (Receptor Activity Modifying Protein1)
Gene Names
RAMP1; RAMP1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
RAMP1; Polyclonal Antibody; Anti - RAMP1 (Receptor Activity Modifying Protein1); RAMP1 (Receptor Activity Modifying Protein1); anti-RAMP1 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The peptides are 52% and 91% conserved in mouse and rat RAMP1, respectively. However, no significant sequence homology is detected with other RAMPs. The control peptide, because of its small size (~2kD), is not suitable for Western. It should be used in ELISA, Dot Blot or to neutralize antibody and confirm specificity of antibody
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
148
Applicable Applications for anti-RAMP1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 19 AA Peptide mapping at the middle and an 11 aa peptide mapping near the C-terminus of human RAMP1 (1) were selected for antibody production. The peptides were coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.d sections of tissues may be tested.
Related Product Information for anti-RAMP1 antibody
IntroductionThe calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175 aa; rat 182 aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung.
Product Categories/Family for anti-RAMP1 antibody
References
• (1). McLatchie LM et al (1998) Nature 393, 333-339.
• 2. Nagae T et al (2000) BBRC 270, 89-93.
• 3. Chakravarty p et al (2000) Br J. Pharmacol. 130, 189-195.
• 4. Aldecoa A et al (2000) FEBS Lett. 471, 156-160.
• 5. Husmann, K et al (2000); Mol Cell Endocrinol (2000) 162, 35-43
• 2. Nagae T et al (2000) BBRC 270, 89-93.
• 3. Chakravarty p et al (2000) Br J. Pharmacol. 130, 189-195.
• 4. Aldecoa A et al (2000) FEBS Lett. 471, 156-160.
• 5. Husmann, K et al (2000); Mol Cell Endocrinol (2000) 162, 35-43
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,988 Da
NCBI Official Full Name
receptor activity-modifying protein 1
NCBI Official Synonym Full Names
receptor (G protein-coupled) activity modifying protein 1
NCBI Official Symbol
RAMP1
NCBI Official Synonym Symbols
RAMP1
NCBI Protein Information
receptor activity-modifying protein 1; OTTHUMP00000164475; CRLR activity-modifying protein 1; receptor activity modifying protein 1; receptor (calcitonin) activity modifying protein 1; calcitonin receptor-like receptor activity modifying protein 1; calcitonin-receptor-like receptor activity-modifying protein 1
UniProt Protein Name
Receptor activity-modifying protein 1
Protein Family
UniProt Gene Name
RAMP1
UniProt Entry Name
RAMP1_HUMAN
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Product Notes
The RAMP1 ramp1 (Catalog #AAA616508) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - RAMP1 (Receptor Activity Modifying Protein1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAMP1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using ECL. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the RAMP1 ramp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAMP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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