Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-435 cells treated with PMA using Raf1 (phospho-Ser338) antibody)
Rabbit RAF1 Polyclonal Antibody | anti-RAF1 antibody
RAF1 Antibody (Ser338)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
RAF1; Polyclonal Antibody; RAF1 Antibody (Ser338); Rabbit Polyclonal RAF1 Antibody (Ser338); Polyclonal RAF1 antibody; Anti-RAF1 antibody; c-Raf antibody; cRaf antibody; NS5 antibody; Oncogene RAF1 antibody; Proto-oncogene c-RAF antibody; RAF antibody; raf proto-oncogene serine/threonine protein kinase antibody; RAF proto-oncogene serine/threonine-protein kinase antibody; Raf-1 antibody; RAF1 antibody; v-raf-1 murine leukemia viral oncogene homolog 1 antibody; anti-RAF1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Raf1 antibody (Ser338) detects endogenous levels of Raf1 only whenphosphorylated at serine 338
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
421
Applicable Applications for anti-RAF1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1000
Immunogen
Raf1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Raf1 around the phosphorylation site of serine 338 (R-D-SP-S-Y) as the immunogen.
Cross-Reactivity
Mouse
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-435 cells treated with PMA using Raf1 (phospho-Ser338) antibody)
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-435 cells treated with PMA using Raf1 (phospho-Ser338) antibody)
Related Product Information for anti-RAF1 antibody
Raf1 is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.
Product Categories/Family for anti-RAF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
RAF1, partial
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase
Protein Family
NCBI Description
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Research Articles on RAF1
Similar Products
Product Notes
The RAF1 (Catalog #AAA5313633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1000. Researchers should empirically determine the suitability of the RAF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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