Rabbit RAD51 Polyclonal Antibody | anti-RAD51 antibody

Anti-RAD51 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100925

• Gene Names: RAD51; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: RAD51; Polyclonal Antibody; Anti-RAD51 Antibody; Rabbit Polyclonal; RAD51 Antibody; Rabbit PAb; Antigen Affinity Purified; RAD51 recombinase; Anti-BRCC5 Antibody; Anti-HRAD51 Antibody; Anti-HsRad51 Antibody; Anti-HsT16930 Antibody; Anti-MRMV2 Antibody; Anti-RAD51A Antibody; Anti-RECA Antibody; anti-RAD51 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RAD51
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RAD51 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human RAD51
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human RAD51, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human RAD51 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAD51 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of RAD51 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RAD51 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-RAD51 antibody

RAD51 recombinase assembles on single-stranded (ss)DNA substrates exposed by DNA end-resection to initiate homologous recombination (HR), a process fundamental to genome integrity. The human RAD51 recombinase possesses DNA pairing and strand exchange activities that are essential for the error-free, homology-directed repair of DNA double-strand breaks. The recombination activities of RAD51 are activated upon its assembly into presynaptic filaments on single-stranded DNA at resected DSB ends. RAD51 recombinase plays a critical role for cancer cell proliferation and survival. Targeting RAD51 is therefore an attractive strategy for treating difficult-to-treat cancers, e.g. triple negative breast cancers which are often resistant to existing therapeutics.RAD51 recombinase polymerizes at the site of double-strand breaks (DSBs) where it performs DSB repair. The loss of RAD51 causes extensive chromosomal breaks, leading to apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 256017143
• NCBI GeneID: 5888
• NCBI Accession #: NP_001157741.1
• NCBI GenBank Nucleotide #: NM_001164269.1
• UniProt Accession #: Q06609; Q6FHX9; Q6ZNA8; Q9BV60; B0FXP0; B2R8T6
• Molecular Weight: 36,966 Da
• NCBI Official Full Name: DNA repair protein RAD51 homolog 1 isoform 2
• NCBI Official Synonym Full Names: RAD51 homolog (S. cerevisiae)
• NCBI Official Symbol: RAD51
• NCBI Official Synonym Symbols: RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• NCBI Protein Information: DNA repair protein RAD51 homolog 1; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; BRCA1/BRCA2-containing complex, subunit 5
• UniProt Protein Name: DNA repair protein RAD51 homolog 1
• Protein Family: RAD51-like protein
• UniProt Gene Name: RAD51
• UniProt Synonym Gene Names: RAD51A; RECA; HsRAD51; hRAD51
• UniProt Entry Name: RAD51_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

RAD51: a member of the RAD51 protein family. Involved in the homologous recombination and repair of DNA. Interacts with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Interacts with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both its intracellular localization and DNA-binding ability. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Defects in RAD51 are associated with breast cancer. Two alternatively spliced isoforms have been reported.

Protein type: DNA repair, damage; Mitochondrial

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: nuclear chromosome; PML body; condensed nuclear chromosome; mitochondrion; condensed chromosome; nucleoplasm; lateral element; mitochondrial matrix; perinuclear region of cytoplasm; cytoplasm; nucleolus; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; double-stranded DNA binding; damaged DNA binding; single-stranded DNA binding; ATP binding; single-stranded DNA-dependent ATPase activity

Biological Process: DNA unwinding during replication; meiosis; mitotic recombination; DNA recombinase assembly; meiotic recombination; double-strand break repair; positive regulation of DNA ligation; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination; protein homooligomerization; DNA recombination

Disease: Breast Cancer; Mirror Movements 2