Rabbit anti-Human, Mouse Rad23A Polyclonal Antibody | anti-Rad23A antibody

Rad23A Polyclonal Antibody

Cat. Num. AAA3010066

• Gene Names: RAD23A; HR23A; HHR23A
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rad23A; Polyclonal Antibody; Rad23A Polyclonal Antibody; UV excision repair protein RAD23 homolog A; HR23A; hHR23A; RAD23A; anti-Rad23A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rad23A polyclonal antibody detects endogenous levels of Rad23A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 362

• Applicable Applications for anti-Rad23A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human Rad23A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of Rad23A polyclonal antibody)

Related Product Information for anti-Rad23A antibody

The RAD23 genes (RAD23A and RAD23B), which encode the human Rad23 proteins, are crucial for excision-repair of UV-damaged DNA. RAD23 genes resemble the other DNA repair genes, RAD2, RAD6, RAD7, RAD18 and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Rad23 is a nuclear protein containing an ubiquitin-like domain required for biological functions. The protein, which is highly conserved, is involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. The C-terminal ubiquitin-associated domain of Rad23 is evolutionarily conserved from yeast to humans. Rad23 may also act as a regulator for the activity of the 26S Proteasome.

NCBI and Uniprot Product Information

• NCBI GI #: 392996949
• NCBI GeneID: 5886
• NCBI Accession #: NP_001257291.1
• NCBI GenBank Nucleotide #: NP_001257291.1
• UniProt Accession #: P54725
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: UV excision repair protein RAD23 homolog A isoform 2
• NCBI Official Synonym Full Names: RAD23 homolog A, nucleotide excision repair protein
• NCBI Official Symbol: RAD23A
• NCBI Official Synonym Symbols: HR23A; HHR23A
• NCBI Protein Information: UV excision repair protein RAD23 homolog A
• UniProt Protein Name: UV excision repair protein RAD23 homolog A
• Protein Family: UV excision repair protein
• UniProt Gene Name: RAD23A
• UniProt Synonym Gene Names: HR23A; hHR23A
• UniProt Entry Name: RD23A_HUMAN

NCBI Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

RAD23A: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Belongs to the RAD23 family.

Protein type: DNA repair, damage; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: proteasome complex; nucleoplasm; cytoplasm

Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of viral genome replication; viral reproduction; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process