Rabbit anti-Human RAD23A Polyclonal Antibody | anti-RAD23A antibody

RAD23A, CT (UV Excision Repair Protein RAD23 Homolog A, HHR23A) (APC)

Cat. Num. AAA6496530

• Gene Names: RAD23A; HR23A; HHR23A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: RAD23A; Polyclonal Antibody; CT (UV Excision Repair Protein RAD23 Homolog A; HHR23A) (APC); anti-RAD23A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAD23A.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RAD23A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human RAD23A (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RAD23A Antibody (C-term) in HL60 cell line lysates (35ug/lane). RAD23A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-RAD23A antibody

Antibodies; Transcription Factors

References

1. Mueller, T.D., et al., EMBO J. 22(18):4634-4645 (2003). 2. Mueller, T.D., et al., J. Mol. Biol. 319(5):1243-1255 (2002). 3. Elder, R.T., et al., Nucleic Acids Res. 30(2):581-591 (2002). 4. Chen, L., et al., EMBO Rep. 2(10):933-938 (2001). 5. Gaynor, E.M., et al., Exp. Cell Res. 267(2):243-257 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 392996949
• NCBI GeneID: 5886
• NCBI Accession #: NP_001257291.1
• NCBI GenBank Nucleotide #: NM_001270362.1
• UniProt Accession #: P54725
• Molecular Weight: 39,609 Da
• NCBI Official Full Name: UV excision repair protein RAD23 homolog A isoform 2
• NCBI Official Synonym Full Names: RAD23 homolog A (S. cerevisiae)
• NCBI Official Symbol: RAD23A
• NCBI Official Synonym Symbols: HR23A; HHR23A
• NCBI Protein Information: UV excision repair protein RAD23 homolog A; RAD23, yeast homolog, A
• UniProt Protein Name: UV excision repair protein RAD23 homolog A
• Protein Family: UV excision repair protein
• UniProt Gene Name: RAD23A
• UniProt Synonym Gene Names: HR23A; hHR23A
• UniProt Entry Name: RD23A_HUMAN

NCBI Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

RAD23A: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Belongs to the RAD23 family.

Protein type: DNA repair, damage; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: proteasome complex; nucleoplasm; cytoplasm

Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of viral genome replication; viral reproduction; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process